A Metabolomic Approach to Probing Myocardial Fuel Shifts in Human Heart Failure

University of Florida

Status: Withdrawn

Conditions: Heart Failure

Treatments: Other: Blood Drawn

Study type: Observational

Funder types: Other

Identifiers

NCT02369913

201401007

Details and patient eligibility

About

This proposal is designed to test the hypothesis that humans shift to reliance on ketone bodies as a cardiac fuel source during the development of HF. The investigator also propose that this shift to ketone utilization can be detected by metabolomic profiling of blood.

Specific Aim 1: To determine if the failing human heart exhibits increased extraction of blood-borne ketone bodies. Ketone bodies will be measured in coronary sinus, arterial, and central venous blood samples to determine AV differences. The HF group will be comprised of patients undergoing cardiac resynchronization therapy with an implantable cardioverter-defibrillator. The Control group will be compromised of patients without evidence of structural heart disease who are undergoing catheter ablation for supraventricular tachycardia.

Specific Aim 2: To determine whether blood samples from humans with HF display metabolomic signatures indicative of increased myocardial ketone body utilization. Quantitative targeted metabolomics will be performed on the samples described in Aim 1 to determine if metabolite markers (e.g. C4-OH acylcarnitine, acetylcarnitine, succinate) found to be associated with increased myocardial ketone body oxidation in animal models are increased in the human HF group compared to the controls.

Full description

After subjects agree to participate in this study and are scheduled to have cardiac resynchronization therapy, the research personnel will take 1 teaspoon of blood before and after the scheduled cardiac procedure for comparison.

Once subjects agree to participate in this study and are scheduled to have a catheter ablation procedure, research personnel will take 1 teaspoon of blood during the scheduled cardiac procedure.

These samples will be taken from catheters already placed during the scheduled cardiac procedure.

Blood will be sent to a lab at the Sanford-Burnham Metabolomics Core Facility in Orlando, Florida for analysis.

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Undergoing cardiac resynchronization therapy
• Undergoing sinus cannulation

Exclusion criteria

• NYHA class II-IV heart failure

Trial design

0 participants in 2 patient groups

Heart failure subjects

Description:

Heart failure subjects undergoing cardiac resynchronization will have a blood drawn for this study.

Treatment:

Other: Blood Drawn

Heart arrhythmia patients

Description:

Heart arrhythmia patients undergoing ablation will have a blood drawn for this study.

Treatment:

Other: Blood Drawn