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Phase 2a Multi-Center Prospective, Randomized Trial to Evaluate the Safety & Efficacy of Topical PEP-TISSEEL for Diabetic Foot Ulcers (DFU)

R

Rion LLC

Status and phase

Enrolling
Phase 2

Conditions

Diabetic Foot Ulcer

Treatments

Biological: PEP (Purified Exosome Product) / TISSEEL

Study type

Interventional

Funder types

Other
Industry

Identifiers

NCT06319287
PRO-00125

Details and patient eligibility

About

A Phase 2a Multi-Center, Prospective, Randomized, Controlled Study to Evaluate the Safety and Efficacy of Topically Applied PEP-TISSEEL in Subjects with Diabetic Foot Ulcers (DFU)

Full description

The objective of this multi-center, prospective, randomized controlled study is to evaluate the safety and efficacy of PEP-TISSEEL+ Standard of Care (SOC) compared to SOC only treatment in 40 subjects (20 subjects PEP-TISSEEL + SOC and 20 subjects SOC only), specifically for the treatment of their non-healing DFU.

Treatment for the PEP-TISSEEL+SOC arm is:

  • PEP-TISSEEL
  • Mepitel dressing followed by Tegaderm®
  • 3M Cavilon® (may be used on the borders prior to placing the Tegaderm®)
  • Padded 3-layer secondary outer layer dressing (Profore (Smith and Nephew (Memphis, TN)); and
  • Offloaded with an offloading Controlled Ankle Movement (CAM) Boot (FootDefender (Miami, FL) or Total Contact Cast (TCC))

Treatment for the Standard of Care (SOC) only arm is:

  • Fibracol
  • Mepitel dressing followed by Tegaderm® (or equivalent)
  • 3M Cavilon® (may be used on the borders prior to placing the Tegaderm® or equivalent)
  • Padded 3-layer secondary outer layer dressing (Profore (Smith and Nephew (Memphis, TN)) or equivalent); and
  • Offloaded with an offloading CAM Boot (FootDefender (Miami, FL) or TCC)

Enrolled subjects will be randomized in a 1:1 ratio with 20 subjects receiving 2 vials PEP delivered in 10 mL TISSEEL plus SOC and the other 20 subjects receiving SOC only.

Before randomization, subjects must complete a two (2) week run-in period (Screening) in which they will be evaluated for inclusion and exclusion criteria and will receive standard of care treatment with required off-loading CAM Boot (FootDefender, Miami FL) or TCC. The Investigator will choose and document the appropriate type of off-loading modality based on their clinical judgement and the subject's diabetic foot ulcer off-loading requirements.

Enrollment

40 estimated patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

  1. Males and females ≥ 18 years of age

  2. Properly obtained written informed consent

  3. Documented history of Type I or Type II Diabetes Mellitus, requiring oral and/or insulin replacement therapy

  4. The index ulcer is classified as Wagner grade 1 ulcer and remains Wagner 1 Grade between Screening and Randomization/Baseline visit (Visit 1 through Visit 3)

  5. These ulcers are superficial, full-thickness ulcers limited to the dermis, not extending to the subcutaneous tissue

  6. Area of index ulcer must be between 1 cm2 to 15 cm2 post debridement at screening and baseline

  7. The index ulcer must be located anatomically on the foot with ≥ 50% of the wound area below the medial or lateral malleolus

  8. Presence of a persistent nonhealing DFU for at least 4 weeks from Randomization and not more than 1 year that has failed to respond to SOC at any point during this timeframe

  9. Adequate vascular perfusion as evidenced by one of the following:

    1. Dorsal transcutaneous oxygen measurement (TCOM/TcPO2) measurement of

      ≥ 40 mmHg within 90 days of Screening (Visit 1 or 2)

    2. Ankle Branchial Index (ABI) between 0.7 and 1.3 within 90 days of Screening (Visit 1 or 2) using the extremity on which the index ulcer is located

    3. Arterial Doppler ultrasound evaluating for biphasic or triphasic dorsalis pedis and/or posterior tibial vessels at the level of the ankle or a TBI (Toe Brachial Index) of > 0.6 is acceptable within 90 days of Screening (Visit 1 or 2)

  10. The index ulcer has been offloaded with protocol defined offloading device during Screening (Run-In) period through Randomization/Baseline visit.

  11. Must meet one of the following criteria:

    a. Female subjects of Non-Child-Bearing Potential defined as: i. Postmenopausal for at least 1 year (Subject verbal confirmation acceptable), or surgically sterilized (i.e., hysterectomy or bilateral oophorectomy more than 3 months prior to Screening (Visit 1 or 2)), or ii. Bilateral tubal ligation more than 6 months prior to Screening (Visit 1 or 2), or iii. Must have a negative serum β-hCG pregnancy test at Visit 1 and not be breastfeeding prior to being administered with the study drug b. Male subjects of Non-Childbearing Potential are defined as those vasectomized subjects whose vasectomy was performed 6 months prior to Screening (Visit 1 or 2) or those diagnosed as sterile by a physician c. Females and Males of Childbearing Potential who practice an acceptable method of contraception defined as the use of any form of hormonal contraceptive, a barrier method with spermicide, condoms, intrauterine device, or abstinence from sexual intercourse starting at least 60 days prior to Screening and continuing at least 30 days following the last treatment.

    Female will undergo a negative serum β-hCG pregnancy test at Visit 1 and an additional urine test at Baseline/Randomization (Visit 3 or Day 0) and must not be breastfeeding prior to being administered with the study drug

  12. Ability to comply with the study protocol as per investigator discretion

Exclusion criteria

  1. Actively undergoing chemotherapy treatment (localized radiation treatment is allowed if it is not on the DFU wound site and in remission based on scans, bloodwork or some other kind of test, such as a breast biopsy or a bone marrow biopsy)

  2. Ulceration with exposed tendon, capsule, or bone

  3. Suspicion of bone or joint infection by clinical or other criteria as per STONEES criteria below:

    a. STONEES criteria for infection: i. size increase, ii. temperature elevation, iii. os (probe to bone) iv. new areas of breakdown v. exudative vi. erythema/edema vii. smell

  4. Unable or unwilling to utilize the protocol defined offloading device

  5. Subjects who have undergone endovascular or open revascularization of the index limb within the last 30 days from Screening.

  6. Index ulcer has decreased in area by ≥ 30% between Screening (Visit 1) and Baseline visits

  7. Any subject that is currently on/or requires oral, systemic or topical antibiotics, or is anticipated to require their use during the course of the study

  8. Any subject that has vascular compromise requiring surgical intervention or has undergone vascular reconstruction or angioplasty less than 1 month prior to randomization. Any planned surgical procedures during the study participation

  9. Serum Creatinine level > 3.0 mg/dL

  10. Hemoglobin A1c (HbA1c) >12%

  11. Aspartate Aminotransferase (AST, GOT) and/or Alanine Aminotransferase (ALT, GPT) >3x the upper limit of normal

  12. Acute active Charcot foot

  13. The location of the index ulcer is within 2 cm of any other ulcer

  14. Any subject that would be unable to safely monitor the infection status of the index ulcer at home and return for scheduled visits

  15. History of immunosuppression or taking immunosuppressive agents including systemic corticosteroids, except stable daily doses of 5 mg/day or less for chronic conditions for up to 5 days

  16. Any subject with a life expectancy ≤ 6 months

  17. Pregnancy, including a positive pregnancy test at Baseline, or lactation/breastfeeding at anytime

  18. Use of investigational drugs or biologics within 28 days prior to screening (Visit 1 or 2)

  19. History of a concurrent condition that, in the Investigator's opinion, would jeopardize the safety of the subject or compliance with the protocol

  20. Known or suspected active abuse of alcohol, or non-prescription drugs

  21. Participation in another interventional clinical study or study in the past 30 days of Screening (Visit 1 or 2) or concurrent participation in another interventional clinical study

  22. Subjects who have untreated Hep C

  23. Subjects who are HIV positive

  24. Subjects on anticoagulation that are not maintained within the International Normalized Ratio (INR) of > 3.0

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Parallel Assignment

Masking

None (Open label)

40 participants in 2 patient groups

PEP-TISSEEL+SOC
Active Comparator group
Description:
Treatment for the PEP-TISSEEL+SOC arm is: * PEP-TISSEEL * Mepitel dressing followed by Tegaderm® (Mepilex can be used if subjects are allergic to Tegaderm) * 3M Cavilon® (may be used on the borders prior to placing the Tegaderm or Mepilex) * Padded 3-layer secondary outer layer dressing (Profore (Smith and Nephew (Memphis, TN)); and * Offloaded with an offloading Controlled Ankle Movement (CAM) Boot (Foot Defender (Miami, FL) or Total Contact Cast (TCC)) Use of an alternate offloading device (e.g., Charcot Restraint Orthotic Walker (CROW) boot, custom shoe, etc.) may be approved on a case-by-case basis
Treatment:
Biological: PEP (Purified Exosome Product) / TISSEEL
Standard of Care
Sham Comparator group
Description:
* Fibracol * Mepitel dressing followed by Tegaderm (Mepilex can be used if subjects are allergic to Tegaderm) * 3M Cavilon (may be used on the borders prior to placing the Tegaderm or Mepilex ) * Padded 3-layer secondary outer layer dressing (Profore (Smith and Nephew (Memphis, TN)) or equivalent); and * Offloaded with an offloading CAM Boot (Foot Defender (Miami, FL) or TCC) * Use of an alternate offloading device (e.g., Charcot Restraint Orthotic Walker (CROW) boot, custom shoe, etc.) may be approved on a case-by-case basis
Treatment:
Biological: PEP (Purified Exosome Product) / TISSEEL

Trial contacts and locations

1

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Central trial contact

Shariq Khan, M.S.; Maureen Merrifield, Ph.D.

Data sourced from clinicaltrials.gov

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