A Proposed Study of Atherosclerotic Plaques in Leg Arteries

Southwest Regional Wound Care Center

Status: Completed

Conditions: Diabetes; Vascular Disease

Study type: Observational

Funder types: Other

Identifiers

NCT01803126

56-RW-024

Details and patient eligibility

About

Biofilm has been identified as the major bacterial phenotype contributing to atherosclerosis. It has become very important to evaluate atherosclerosis and the role of biofilm using advanced technologies. It is also important to understand wound biofilm at a genetic and a molecular level.

Full description

High throughput pyrosequencing is opening new doors in nucleic acid analysis. 454 Roche has developed sequencing instrumentation which has the ability to sequence not only genomic DNA (gDNA) but also derivatives of messenger RNA (mRNA) and 16s ribosomal DNA (rDNA). This technology has the potential to survey the nucleic acids involved within a mixed population of species, such as atherosclerotic biofilm. In addition to transcriptome science; proteome, metabolome, and other -omic sciences are advancing rapidly. Examining atherosclerotic plaque using transcriptome, proteome, metabolome, and other -omic approaches may provide valuable insight into the wound's microecology.

Enrollment: 10 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

1. The subject with identifying leg or legs that were amputated for vascular disease and/or diabetes.
2. The subject must be 18 years of age or older.

Exclusion criteria

• The subject will be excluded if they have had a traumatic amputation which did not involve vascular disease and/or diabetes.

Trial design

10 participants in 1 patient group

atherosclerosis

Description:

No treatment.