A Prospective Study Comparing the Outcomes of Single Versus Group Culture in Preimplantation Genetic Testing Cycles

Reproductive & Genetic Hospital of CITIC-Xiangya

Status: Unknown

Conditions: Embryo Culture

Treatments: Other: embryo culture

Study type: Interventional

Funder types: Other

Identifiers

NCT03464643

KYXM-20180307

Details and patient eligibility

About

The aim of this study is to analyze whether there is difference in the effect of single embryo culture and group culture in preimplantation genetic screening (PGS) cycle, and provide reference for the feasibility of the clinical application of medium-based noninvasive PGD. This prospective study prepares for more than 1,000 normal fertilized zygotes in each group over one year period. For the recruited patients, all normal fertilized zygotes were randomly allocated into two groups: cultured individually in 25 ul media or 2-3 embryos cultured together in 50 ul media. The blastocyst formation rates, aneuploid rates, clinical pregnancy rates, implantation rates and miscarriage rates will be compared between the two groups.

Enrollment: 236 estimated patients

Sex: Female

Ages: 20 to 45 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• patients treated by preimplantation genetic diagnostic / screening cycles

Exclusion criteria

• number of normal fertilization zygotes is less than 6

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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236 participants in 2 patient groups

Single embryo culture

Other group

Description:

Embryo is cultured individually in 25 ul media

Treatment:

Other: embryo culture

Group embryo culture

Other group

Description:

2-3 Embryos are group cultured in 50 ul media

Treatment:

Other: embryo culture