A Study of Adipose Tissue in Adaptive Responses to Exercise

Mayo Clinic

Status: Active, not recruiting

Conditions: Healthy; Obesity

Treatments: Procedure: Adipose Tissue Biopsy; Behavioral: Exercise

Study type: Interventional

Funder types: Other; NIH

Identifiers

NCT06053125

23-002049

K01DK134765 (U.S. NIH Grant/Contract)

Details and patient eligibility

About

The purpose of this research is to determine how exercise affects fat (adipose) tissue and how changes to adipose tissue that occur during and after exercise might improve health in aging and obesity.

Full description

Adipose tissue has important endocrine functions that influence metabolic health. Early evidence shows that adipose adapts to physiological stress, including exercise. The objective here is to determine how exercise-induced alterations in adipose tissue cellular composition and endocrine signaling may contribute to the beneficial adaptations to exercise in aging and obesity.

Immune cell populations and inflammatory signatures will be assessed in subcutaneous abdominal adipose tissue biopsies collected from obese and normal weight young and older adults before, immediately after, and 3 hours after a 30-min bout of cycling exercise at 70% of maximal oxygen consumption (VO2max). Mass spectrometry, Olink targeted inflammation assays, and RNA sequencing will be used for full proteomic and transcriptomic characterization of the adipose tissue secretome (the proteins and molecules secreted from the adipose tissue) and the cargo of extracellular vesicles isolated from plasma and media collected from cultured human adipose tissue explants generated from each time point.

Overall, the primary hypothesis of the proposed work is that a single bout of exercise triggers transient changes in adipose tissue paracrine/endocrine signals and immune cellular composition. The investigators propose that these responses contribute to the beneficial effects of exercise locally and in distal tissues and that the cumulative effects of acute changes in adipose tissue likely contribute to the positive alterations in adipose tissue associated with exercise training.

Enrollment: 56 patients

Sex: All

Ages: 18 to 85 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Adults between the ages of 18-35 OR 65-85.
• BMI 18.5 kg/m2 - 40 kg/m2
• Weight stable (≥ 3 months)
• Generally healthy

Exclusion criteria

• Participation in ≥ 30 minutes of structured physical activity ≥ 2 days per week.
• Smoking/tobacco use.
• Alcohol/substance abuse.
• Pregnancy and breastfeeding.
• Anemia.
• Abnormal renal function.
• Blood clotting disorders.
• Coronary artery disease.
• Uncontrolled thyroid disease.
• Liver disease.
• Use of medications known to influence the main outcomes of the study.
• Orthopedic problems that may be aggravated by exercise.
• Chronic disease at the discretion of the investigators.

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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56 participants in 4 patient groups

Older adults with obesity

Experimental group

Description:

Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.

Treatment:

Behavioral: Exercise

Procedure: Adipose Tissue Biopsy

Older adults without obesity

Experimental group

Description:

Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.

Treatment:

Behavioral: Exercise

Procedure: Adipose Tissue Biopsy

Young adults with obesity

Experimental group

Description:

Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.

Treatment:

Behavioral: Exercise

Procedure: Adipose Tissue Biopsy

Young adults without obesity

Experimental group

Description:

Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.

Treatment:

Behavioral: Exercise

Procedure: Adipose Tissue Biopsy