Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome (IBS)

Chinese PLA General Hospital (301 Hospital)

Status: Unknown

Conditions: Irritable Bowel Syndrome; Gastrointestinal Diseases; Intestinal Diseases; Digestive System Diseases; Colonic Diseases; Colonic Diseases, Functional

Study type: Observational

Funder types: Other

Identifiers

NCT01028898

IBS and metabolism

Details and patient eligibility

About

In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

Full description

For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.

Enrollment: 180 estimated patients

Sex: All

Ages: 18 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients with symptoms meeting Rome III criteria for diagnosis of IBS-C and IBS-D

Exclusion criteria

• Patients with organic illnesses or a history of major abdominal surgery
• Major psychotic episode, mental retardation or dementia
• Pregnant women and lactational women
• Postinfectious IBS, PI-IBS