Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome (IBS)

C

Chinese PLA General Hospital (301 Hospital)

Status

Unknown

Conditions

Irritable Bowel Syndrome
Gastrointestinal Diseases
Intestinal Diseases
Digestive System Diseases
Colonic Diseases
Colonic Diseases, Functional

Study type

Observational

Funder types

Other

Identifiers

NCT01028898
IBS and metabolism

Details and patient eligibility

About

In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

Full description

For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.

Enrollment

180 estimated patients

Sex

All

Ages

18 to 60 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Patients with symptoms meeting Rome III criteria for diagnosis of IBS-C and IBS-D

Exclusion criteria

  • Patients with organic illnesses or a history of major abdominal surgery
  • Major psychotic episode, mental retardation or dementia
  • Pregnant women and lactational women
  • Postinfectious IBS, PI-IBS

Trial contacts and locations

0

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Data sourced from clinicaltrials.gov

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