ACTH Stability on Whole Blood (STABACT)

University Hospital, Rouen

Status: Unknown

Conditions: ACTH

Treatments: Biological: Blood sampling

Study type: Interventional

Funder types: Other

Identifiers

NCT04266587

2019/0350/HP

Details and patient eligibility

About

ACTH is a peptide secreted by pituitary gland and plays an important role in regulating cortisol secretion. ACTH is determined in plasma by immunoassays using specific antibodies. Its determination is difficult because of instability in whole blood. Several factors which influence ACTH stability in blood before analysis have been identified: temperature, hemolysis, time to centrifugation and presence of protease inhibitors. Published results on ACTH whole blood stability seem contradictory.

The objective of this study is to evaluate the effect of aprotinin in 10 healthy volunteers. ACTH measurements will be performed on cobas e602 (Roche Diagnostics, Mannheim, Germany).

Enrollment: 10 estimated patients

Sex: All

Ages: 18 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Adult patient aged 18 to 60,

Exclusion criteria

• Adult with insufficient venous capital for blood collection

Trial design

Primary purpose: Other

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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10 participants in 1 patient group

Healthy volunteers

Experimental group

Description:

blood sampling is done on healthy volunteers

Treatment:

Biological: Blood sampling