Activation of miRNA and Proteins After Acute Experimental Pain. (miRNA: Micro RNA)

Aalborg University

Status: Completed

Conditions: Hypertonic Saline; Isotonic Saline

Treatments: Other: Saline injection; Procedure: Blood sampling; Other: hypertonic saline

Study type: Interventional

Funder types: Other

Identifiers

NCT04439994

N-20200015

Details and patient eligibility

About

In this experiment, the investigators would like to test the following:

1. Does acute pain induced by hypertonic injection cause changes in plasma miRNA and protein expression ?
2. Can these canges correlate with the development of local hyperalgesia?
3. How does the plasma miRNA expression change over a duration of 24 hours post pain induction ?
4. Which mRNA targets are potentially affected by acute pain?

Enrollment: 20 patients

Sex: All

Ages: 18 to 80 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Healthy men and women in the age 18-80 years
• Speak and understand English

Exclusion criteria

• Acute and chronic pain
• Pregnancy or breastfeeding
• Drug addiction defined as the use of cannabis, opioids or other drugs
• Present or previous history of neurological, dermatological, immunological, musculoskeletal, cardiac disorder or mental illnesses that may affect the results (e.g. Neuropathy, muscular pain in the upper extremities, etc.)
• Lack of ability to cooperate
• Current use of medications that may affect the trial, such as antipsychotics and pain killers as well as systemic or topical steroids and anti-inflammatory drugs.
• Skin diseases
• Consumption of alcohol or painkillers 24 hours before the study days and between these
• Participation in other trials within 1 week of study entry (4 weeks in the case of pharmaceutical trials)

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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20 participants in 2 patient groups, including a placebo group

Hypertonic saline

Active Comparator group

Description:

Each participant will be given i.d. in 0.1 mL volumes of a hypertonic saline solution. The subject will be blinded to the hypertonic/isotonic administration.

Treatment:

Procedure: Blood sampling

Other: hypertonic saline

Isotonic Saline

Placebo Comparator group

Description:

Each participant will be given i.d. in 0.1 mL volumes of a isotonic saline solution. The subject will be blinded to the hypertonic/isotonic administration.

Treatment:

Other: Saline injection

Procedure: Blood sampling