Acute Effects of Beetroot Juice and Caffeine Co-ingestion on Lower-limb Strength and Muscular Endurance in Females

Jose Manuel Jurado Castro

Status: Completed

Conditions: Caffeine; Beetroot Juice with Caffeine Co-ingestion; Placebo; Beetroot Juice

Treatments: Dietary Supplement: Beetroot juice placebo with caffeine (CAF); Other: Beetroot placebo with caffeine placebo (PLA); Dietary Supplement: Beetroot juice with caffeine placebo (BRJ); Dietary Supplement: Beetroot juice combined with caffeine (COM)

Study type: Interventional

Funder types: Other

Identifiers

NCT06758323

IMIBIC_BRJ_CAF

Details and patient eligibility

About

The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. The main questions it aims to answer are:

• Does the co-ingestion of beetroot juice and caffeine enhance lower-limb strength and muscular endurance more than either substance alone or a placebo?
• How do the acute effects of beetroot juice interact with caffeine on physical performance?

The study was conducted using a randomized, crossover design to evaluate the following four conditions:

Beetroot juice with placebo Caffeine with placebo Beetroot juice with caffeine Placebo only

Each participant completed all conditions, with a sufficient washout period between sessions to eliminate potential carryover effects. This design allowed for a direct comparison of the acute effects of each supplementation strategy on lower-limb strength and muscular endurance.

Participant Protocol

Participants consumed their assigned supplement on a single testing day for each condition. After supplementation, they performed standardized tests to measure lower-limb strength and muscular endurance. Progress was monitored, and data from all conditions were collected to assess the acute effects of each intervention.

Enrollment: 15 patients

Sex: Female

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Minimal experience of six months of strength training
• Be familiarized with the exercise test: counter movement jump (CMJ), barbell back squats, leg press and knee extension.

Exclusion criteria

• Supplementation consumption
• Sports injuries

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Double Blind

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15 participants in 4 patient groups, including a placebo group

COM

Experimental group

Description:

Beetroot juice combined with caffeine

Treatment:

Dietary Supplement: Beetroot juice combined with caffeine (COM)

CAF

Active Comparator group

Description:

Beetroot juice placebo with caffeine

Treatment:

Dietary Supplement: Beetroot juice placebo with caffeine (CAF)

BRJ

Active Comparator group

Description:

Beetroot juice with caffeine placebo

Treatment:

Dietary Supplement: Beetroot juice with caffeine placebo (BRJ)

PLA

Placebo Comparator group

Description:

Beetroot juice placebo with caffeine placebo

Treatment:

Other: Beetroot placebo with caffeine placebo (PLA)