Acute Resistance Exercise and Hydrolyzed Collagen Supplementation

Rob Erskine

Status: Completed

Conditions: Nutrition; Food; Diet

Treatments: Dietary Supplement: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise; Dietary Supplement: Consumption of three different doses of hydrolyzed collagen (HC) with resistance exercise

Study type: Interventional

Funder types: Other

Identifiers

NCT06236659

19/SPS/049

Details and patient eligibility

About

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in middle-aged males and females.

Full description

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy middle-aged males and females.

Healthy middle-aged males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' leg press RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.

This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in middle-aged men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.

Enrollment: 10 patients

Sex: All

Ages: 40 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• At least 12 months experienced in resistance exercise (including weekly lower body exercise)
• Free from musculoskeletal injury

Exclusion criteria

• Having a history of patellar tendon pathology
• Vegan
• Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
• Had a sustained a lower limb injury in the previous six months
• Smoker/vaper
• Having reached menopause (only for middle-aged female participants)

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Triple Blind

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10 participants in 2 patient groups

Intervention for middle-aged men: Consumption of hydrolyzed collagen (HC) with resistance exercise

Experimental group

Description:

Middle-aged male participants consumed one of three different HC doses (0 grams, 15 grams, or 30 grams) with 4 sets of 10 repetitions of leg press exercise at 10-repetition maximum load in a random order and a seven-day wash-out period interspersed between each trial.

Treatment:

Dietary Supplement: Consumption of three different doses of hydrolyzed collagen (HC) with resistance exercise

Intervention for middle-aged women: Consumption of hydrolyzed collagen (HC) with resistance exercise

Experimental group

Description:

The intervention procedure is exactly same as Arm 1 except for the number of visits and doses of HC. Middle-aged female participants were asked to visit the laboratory on the day of highest oestrogen (i.e. ovulation) and provided with 0 g or 30 g HC. Dates for the trials were determined based on self-report of onset of menses and previous menstrual cycle length.

Treatment:

Dietary Supplement: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise