Adding Cytokines to In Vitro Human Culture Media to Improve Embryogenesis and Implantation (GHLCSHED)

Ibn Sina Hospital

Status: Completed

Conditions: Infertility

Treatments: Other: Adding LIF, HB-EGF and GM-CSF to Lifeglobal human IVF culture media

Study type: Interventional

Funder types: Other

Identifiers

NCT02420886

Ibnsina IVF center

Details and patient eligibility

About

Assessment of the embryological benefit of in vitro adding of Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2n/ml) to the culture media and closely monitor its effect on embryogenesis and implantation outcomes.

Full description

The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding the Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2ng/ml) as a growth factor that have multi-ways to might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between two arm of the same culture media and the same culture condition. One arm with Cytokines and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.

Enrollment: 443 patients

Sex: Female

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• all patients

Exclusion criteria

• over 40 years

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Triple Blind

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443 participants in 2 patient groups

Cytokines supplemented In Vitro single culture media

Active Comparator group

Description:

We will add Cytokines (LIF, HB-EGF and GM-CSF) to LIFEGLOBAL culture media and assess the embryogenesis and pregnancy.

Treatment:

Other: Adding LIF, HB-EGF and GM-CSF to Lifeglobal human IVF culture media

Traditional Single step culture media

No Intervention group