Adding Insulin to IVF Culture Media Significantly Improve Outcome

Ibn Sina Hospital

Status: Completed

Conditions: Infertility

Treatments: Other: Adding insulin to In Vitro culture media

Study type: Interventional

Funder types: Other

Identifiers

NCT02420171

Ibnsina IVF center Sohag

Details and patient eligibility

About

Add insulin to a single step culture media for IVF embryo culture from day 0 to day 5/6 of In Vitro culture and detect the the effect upon embryo quality and implantation potential.

Full description

The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding insulin as a hormon and growth factor might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between to arm of the same culture media and yhe same culture condition but with one arm with insulin and the other is traditional one and closely monitor the outcome within the 2 arms abd record the results

Enrollment: 360 patients

Sex: Female

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• normal responder
• PCOS
• Tubal factor infertility
• mild asthenozoospermia

Exclusion criteria

• patient over 40

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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360 participants in 2 patient groups

In Vitro culture media with insulin

Active Comparator group

Description:

We will add insulin to the single step culture media to monitor the embryogenesis

Treatment:

Other: Adding insulin to In Vitro culture media

In Vitro culture media without insulin m

No Intervention group

Description:

The embryos will be cultured in the media without insulin and compare this arm as the control arm with the interventional one.