Amino Acid Metabolism Before and After Bariatric Surgery

Harran University

Status: Completed

Conditions: Obesity, Morbid

Treatments: Procedure: Blood sample collection for amino acid profiling

Study type: Observational

Funder types: Other

Identifiers

NCT07008352

Gerger

Details and patient eligibility

About

This observational study aims to investigate the changes in serum amino acid metabolism before and after bariatric surgery in obese individuals. Blood samples will be collected from 50 patients at baseline (preoperative), and at 1 and 3 months postoperatively. An additional 50 healthy individuals will serve as the control group. Amino acid profiling will be conducted using LC-MS/MS. The study seeks to determine how bariatric surgery affects metabolic pathways associated with amino acid regulation.

Full description

Obesity is a major global health problem that significantly increases the risk of metabolic disorders. Bariatric surgery is one of the most effective interventions for sustained weight loss in morbidly obese patients. However, the metabolic effects of such surgery on amino acid profiles are not fully understood.

In this prospective observational study, a total of 100 participants will be enrolled: 50 patients undergoing bariatric surgery and 50 healthy controls. Serum samples will be collected from the patient group preoperatively, and at 1 and 3 months after surgery. The control group will provide one-time blood samples.

All serum samples will be centrifuged and stored at -80°C until analysis. Free amino acid profiles will be determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The primary outcome is to assess the change in amino acid concentrations over time in surgical patients, and to compare them with healthy controls. Secondary analyses will explore correlations between amino acid levels and body mass index (BMI). Data analysis will be performed using SPSS and MetaboAnalyst 5.0.

This study will help clarify how bariatric surgery influences amino acid metabolism and may offer insights into the metabolic mechanisms underlying postoperative recovery and weight regulation.

Enrollment: 100 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age ≥18 years
• Diagnosed with obesity and scheduled for bariatric surgery (study group)
• Healthy individuals with normal BMI (control group)

Exclusion criteria

• Age <18 years
• History of metabolic or chronic systemic disease
• Pregnancy or lactation

Trial design

100 participants in 2 patient groups

Group 1: Bariatric surgery patients (n=50)

Description:

Participants in this group are adults (≥18 years old) with obesity who are scheduled to undergo bariatric surgery at Harran University Hospital. Blood samples will be collected at three time points: before surgery (baseline), and at 1 and 3 months postoperatively. Serum will be separated and stored at -80°C for subsequent amino acid profiling using LC-MS/MS.

Treatment:

Procedure: Blood sample collection for amino acid profiling

Group 2: Healthy control participants (n=50)

Description:

Participants in this group are healthy adults (≥18 years old) with no known history of obesity or chronic metabolic disease. A single blood sample will be collected at baseline. Serum samples will be analyzed using LC-MS/MS to determine amino acid profiles, which will be compared to those of the bariatric surgery group.

Treatment:

Procedure: Blood sample collection for amino acid profiling