An in Vitro Evaluation of Antimicrobial Photodynamic Therapy on Deciduous Carious Dentin

University of Sao Paulo

Status and phase: Completed; Phase 1

Conditions: Dental Caries

Treatments: Procedure: Antimicrobial Photodynamic Therapy 1; Procedure: Dentin wash with chlorhexidine 2%; Procedure: Antimicrobial Photodynamic Therapy 2; Procedure: Rubber dam isolation; Procedure: Tooth drying

Study type: Interventional

Funder types: Other

Identifiers

NCT02479958

150/10

Details and patient eligibility

About

Despite of the decline of dental caries, this disease is still common in polarized groups affecting children. Therefore, the aim of this randomized blind in vivo study was to compare the antimicrobial effect of two antimicrobial therapies in deciduous carious dentin. Thirty two patients aging from 5 to 7 years old had partial caries removal in deep carious dentin lesion in deciduous molar and were divided in three groups: 1. Control - chlorhexidine and resin modified glass ionomer cement (RMGIC); 2. LEDTB - antimicrobial photodynamic therapy (APDT) with LED (light emission diode) associated with toluidine blue orto dye solution and RMGIC; 3. LMB - APDT with laser associated with methylene blue dye solution and RMGIC. The patients were submitted to initial clinical and radiographic examination and demographic features were evaluated by biofilm, gingival, and dmft/dmfs indexes, besides clinical and radiographic follow up at 6 and 12 months after the treatments. The carious dentin was collected before and after each treatment and the number of Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Fusobacterium nucleatum, Atopobium rimae and total bacteria were established by quantitative PCR.

Enrollment: 58 patients

Sex: All

Ages: 5 to 7 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Inclusion criteria consisted of good general health, without syndromes or chronic systemic diseases. They should also have at least one primary molar with deep carious lesion (2/3 of dentin) without pain symptomatology and compatible with reversible pulpits.

Exclusion criteria

• Children whose parents refused to sign the informed consent document, who did not cooperate with the clinical exams, whose teeth had the treatment choice changed (pain or evolution to irreversible pulpits), who did not attend to the appointments for the scheduled treatments, or who needed antibiotics treatment for other medical reasons were excluded from the study without prejudice.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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58 participants in 3 patient groups

Control

Experimental group

Treatment:

Procedure: Dentin wash with chlorhexidine 2%

Procedure: Rubber dam isolation

Procedure: Tooth drying

APDT 1

Experimental group

Treatment:

Procedure: Rubber dam isolation

Procedure: Antimicrobial Photodynamic Therapy 1

Procedure: Tooth drying

APDT 2

Experimental group

Treatment:

Procedure: Rubber dam isolation

Procedure: Tooth drying

Procedure: Antimicrobial Photodynamic Therapy 2