Analysis of Expression of Nitric Oxide Syntheses and Their Functional Role in Human Urothelium

Copenhagen University Hospital at Herlev

Status: Terminated

Conditions: Interstitial Cystitis; Bladder Diseases; Urinary Incontinence

Treatments: Procedure: Tissue examination for protein- and mRNA-expression of NO

Study type: Observational

Funder types: Other

Identifiers

NCT00253006

UA_H_Ka-05104-m

Details and patient eligibility

About

Painful bladder syndrome (PBS)/interstitial cystitis (IC) may be due to the actions of nitric oxide (NO) in the bladder tissue. NO is a gaseous substance with a very short half-life, synthesized by a group of NO-synthase-enzymes in many tissues.

The goal of this study is to illuminate bladder tissue production of NO in individuals with PBS and healthy individuals, by quantification of NO and NO-enzyme expression by different molecular biological methods.

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• PBS/IC according to International Continence Society (ICS)
• Cystoscopy with bladder distension and deep biopsies within last 5 years
• Cystometry within last 5 years
• Normal urine culture within last month

Inclusion Criteria (for control group):

• Benign non-invasive bladder disease
• No PBS/IC
• Normal urine culture
• No functional bladder disease of known nature (ex. infravesical obstruction)

Exclusion criteria

• Age under 18 years
• Inability to understand instructions
• Pregnancy and lactation