Analysis of Gingival Crevicular Fluid (GCF)

B

Bezmialem Vakif University

Status

Completed

Conditions

Periodontal Diseases

Treatments

Diagnostic Test: Gingival crevicular fluid sampling

Study type

Observational

Funder types

Other

Identifiers

NCT05325905
2014/01

Details and patient eligibility

About

The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.

Full description

Gingival crevicular fluid (GCF) is formed when fluid exudes from the vessels of the microcirculation into the inflamed periodontal tissue and into the sulcus or pocket. As the fluid traverses the inflamed tissue, it is thought to pick up enzymes and other molecules that participate in the destructive process, as well as products of cell and tissue degradation. Efforts to develop diagnostic tests based on host factors have been focused almost entirely on analysis of GCF. The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status. Systemically healthy and non-smokers 80 participants (30 males and 50 females) who were selected from among admitting to Dentistry Faculty of Selcuk University were included in this study. Clinical periodontal parameters were recorded and GCF samples were obtained. Samples were stored in -80 ˚C until analysis with Enzyme-Linked ImmunoSorbent Assay (ELISA).

Enrollment

81 patients

Sex

All

Ages

20+ years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

  • Systemically healthy
  • non-smokers,
  • presence of at least 20 teeth in the mouth

Exclusion criteria

  • receiving periodontal therapy in the last 6 months;
  • receiving antibiotics or anti-inflammatory drugs in the last 6 months;
  • currently pregnant or breastfeeding
  • using a removable partial denture and removable or fixed orthodontic appliances.

Trial design

81 participants in 3 patient groups

Healthy
Description:
bleeding on probing (BoP) ≤ 10% of the sites, probing depth (PD) ≤ 3 mm and no indication of clinical attachment level (CAL) or radiographic bone loss
Treatment:
Diagnostic Test: Gingival crevicular fluid sampling
Gingivitis
Description:
gingival inflammation (BoP ≥ 10% of the sites, red-blue discoloration of the gingiva and edema) PD ≤ 4 mm and no clinical attachment or radiographic bone loss
Treatment:
Diagnostic Test: Gingival crevicular fluid sampling
Periodontitis
Description:
PD ≤ 5 mm, radiographic bone loss, and 3-4 mm CAL in at least 30% of the sites
Treatment:
Diagnostic Test: Gingival crevicular fluid sampling

Trial contacts and locations

0

Loading...

Data sourced from clinicaltrials.gov

Clinical trials

Find clinical trialsTrials by location
© Copyright 2024 Veeva Systems