Analysis of the Variation in Caspase-8 Availability and Cleavage in Oral Squamous Cell Carcinoma

Guy's and St Thomas' NHS Foundation Trust

Status: Unknown

Conditions: Oral Cancer

Treatments: Other: There is no intervention.

Study type: Observational

Funder types: Other

Identifiers

NCT03877159

232080

Details and patient eligibility

About

Control of cell death is frequently disrupted in cancer resulting in overgrowth of tumour cells. Caspase-8 is a key enzyme involved in controlling cell death. This study examines the importance of caspase-8 in oral cancer.

Full description

Control of cell death is frequently disrupted in cancer resulting in overgrowth of tumour cells. The caspase family of enzymes are the key performers of controlled cell death and caspase-8 is frequently mutated in oral cancer. This study will perform DNA sequencing to determine if the gene is wild-type (not mutated). The abundance of inactive (uncleaved) and active (cleaved) caspase-8 will be determined. The levels of the proteins that caspase-8 interacts with (including caspase-3) will be determined. Results will be correlated with tumour stage, patient outcome and the presence of immune cells and other microscopic features. This will improve the understanding of the importance of caspase-8 in oral cancer.

Enrollment: 100 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Oral cancer
• Sufficient follow up data

Exclusion criteria

• Patient has indicated that they do not wish their tissue to be used for research.
• . The quantity or quality of tissue is too little or poor to provide adequate material without significant reserve.
• The material contains bone.