Angiotensin-converting Enzyme 2 (ACE2) Expression in Tonsils and Adenoids (ACE2-AVG)

Assistance Publique - Hôpitaux de Paris

Status: Withdrawn

Conditions: Adenoidectomy; Tonsillectomy

Treatments: Other: Storage of operating waste

Study type: Observational

Funder types: Other

Identifiers

NCT04640610

APHP201032

Details and patient eligibility

About

The objective of the research is to study the expression of the ACE 2 receptor and the TMPRSS2 serine protease in the tonsils and adenoids of children and adults.

Full description

The entry point for SARS-CoV-2 into the body is the nasal and pharyngeal mucosa. Angiotensin-converting enzyme 2 (ACE 2) was quickly identified as the functional receptor for SARS-CoV-2. Furthermore, the TMPRSS2 serine protease is required for the anchoring of the S protein in cell lines. In addition, the most commonly used screening test is a test for viral RNA by RT-PCR on a nasopharyngeal sample because the viral load is high.

The tonsils and adenoids could be a target organ of the virus. In order to study this hypothesis, the expression of ACE 2 and the serine protease TMPRSS2 will be sought in these tissues.

In addition, children, generally at high risk of severe viral respiratory infections (RSV, influenza), have milder symptoms than older patients with SARS-CoV-2 infection. The reason for this difference remains elusive. But one of the hypotheses formulated is that the level of expression of ACE 2 and / or TMPRSS2 may differ between adults and children, the viral load seeming to be correlated with the severity of the attack. In order to study this hypothesis, we will therefore compare the level of expression of ACE 2 in the tonsil tissue of children and adults.

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• Children and adults, without SARS-CoV-2 infection, in whom an adenoidectomy and/or tonsillectomy is performed for their care in the hospitals of Assistance Publique-Hôpitaux de Paris participating in the study.
• Holders of parental authority of minor patients and adult patients not opposed to participation in the study.

Exclusion criteria

- N/A

Trial design

0 participants in 1 patient group

Adenoidectomy / tonsillectomy

Description:

Children and adults, without SARS-CoV-2 infection, in whom an adenoidectomy and/or tonsillectomy is performed for their care in the centers of the study.

Treatment:

Other: Storage of operating waste