Anti-ficolin-3 Autoantibodies in Lupus Nephritis (ficolupus)

Grenoble Alpes University Hospital Center (CHU)

Status: Completed

Conditions: Lupus Erythematosus, Systemic

Treatments: Other: Biological analysis

Study type: Observational

Funder types: Other

Identifiers

NCT02625831

1841851v0

Details and patient eligibility

About

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the production of multiple autoantibodies. Antibodies against Ficolin-3 were previously identified in the sera of some SLE patients, but their prevalence and significance have not been yet investigated. The aims of this study were to determine the prevalence of anti-ficolin-3 antibodies among SLE patients and to investigate their potential as diagnostic and/or prognostic biomarkers in SLE.

In this retrospective study, clinical data were obtained from medical files and blood samples were selected from preexisting biological collection. SLE patients (n=165) were informed and did not objected, they were matched to healthy controls (n=48). Disease activity was determined according to the SLEDAI score. Anti-ficolin-3, anti-dsDNA and anti-C1q antibodies levels were measured in sera by ELISA. First, a highly significant difference was found in the anti-ficolin-3 levels between SLE patients and healthy subjects. Anti-ficolin-3 antibodies were detected as positive in 58 of 165 (35%) SLE patients. The titer of anti-ficolin-3 antibodies was correlated with the SLEDAI score (p<0.0001). The presence of anti-ficolin-3 antibodies was associated with anti-C1q and anti-dsDNA antibodies. Regarding associations with clinical manifestations, only the presence of active lupus nephritis was significantly associated with the presence of anti-ficolin-3 antibodies (p=0.0001). This association with renal involvement was higher with anti-ficolin-3 antibodies than with other auto-antibodies. Interestingly, the combination of anti-ficolin-3 and anti-C1q antibodies demonstrated higher specificity than any other traditional biomarker.

These results suggest that anti-ficolin-3 could be useful for the diagnosis of active nephritis in SLE patients.

Full description

For this retrospective study, a brief summary should be enough.

Enrollment: 213 patients

Sex: All

Ages: 18 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

165 SLE patients

Inclusion Criteria:

• Age: ≥ 18 years old
• Patients with lupus diagnostic criteria (ACR1997)

Exclusion Criteria:

• Pregnant women

• Patient with known evolutive cancer

  48 healthy patients matched in age and sex with one or several SLE patients

Trial design

213 participants in 2 patient groups

SLE patients

Description:

165 SLE patients. Subgroup : 77 with active lupus and 88 in disease remission. in the active subgroup : 36 with lupus nephritis and 41 without. Biological analysis were performed.

Treatment:

Other: Biological analysis

healthy patients

Description:

48 healthy patients. Biological analysis were performed.

Treatment:

Other: Biological analysis