Anti-inflammatory Cells in Peritoneal Cavity

University Hospital Center (CHU)

Status: Terminated

Conditions: Ovarian Cysts; Chronic Renal Insufficiency

Treatments: Other: peritoneal fluid and blood sample

Study type: Interventional

Funder types: Other

Identifiers

NCT03189316

9828

Details and patient eligibility

About

Some subsets of lymphocytes are able to inhibit immune response and thus, could be used to control auto-immune diseases and transplant reject. In mice, the main source of those anti-inflammatory lymphocytes is the peritoneal cavity. No data are available in human. This study aims at exploring the presence of those anti-inflammatory lymphocytes in human peritoneal cavity and at determine how to expand those cells.

Full description

B cells will be collected during procedures planned for patients' standard care. Peritoneal fluid and peripheral blood of patients will be collected during coelioscopy for exploration of ovarian cysts or dialysis for treatment of chronic renal insufficiency.

Regulatory B cells (Breg cells) will be measured in peritoneal fluid and in peripheral blood after in vitro stimulation and assessment of intracellular interleukin-10 by flow cytometry. Proportion of Breg cells among B cells will be compared between peritoneal fluid and peripheral blood in both groups.

Different protocols to induce differentiation of B cells into Breg cells will be tested and compared.

Enrollment: 10 patients

Sex: All

Ages: 18 to 80 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• woman over 18 year old undergoing exploration of ovarian cysts by coelioscopy
• patient with chronic renal insufficiency undergoing peritoneal dialysis
• informed consent obtained

Exclusion criteria

• pregnancy or breast-feeding
• non affiliation to health care insurance
• treatment with corticosteroids over 15 mg/day
• treatment with rituximab

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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10 participants in 2 patient groups

subjects with ovarian cyst

Other group

Description:

Peritoneal fluid sample obtained from coelioscopy for exploration of ovarian cysts and blood sample

Treatment:

Other: peritoneal fluid and blood sample

subjects with peritoneal dialysis

Other group

Description:

Peritoneal fluid sample obtained from peritoneal dialysis fluid of patients with chronic renal insufficiency and blood sample

Treatment:

Other: peritoneal fluid and blood sample