Antibacterial Activity of Urea Against Ocular Bacteria

Assiut University

Status: Completed

Conditions: Urea in Blood; High

Treatments: Biological: Antibacterial Activity

Study type: Observational

Funder types: Other

Identifiers

NCT04020406

2323297

Details and patient eligibility

About

Antibacterial properties of urea was tested against 35 isolates from ocular infections.

Full description

In vitro susceptibility testing was performed with commercially available antibiotic discs, homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined discs (antibiotic discs to which urea solution was added). Combined discs provided better in vitro susceptibility results than antibiotics alone.

Scanning electron micrographs of bacterial isolates incubated overnight with urea solution showed marked disruption of bacterial aggregative patterns and irregularities of their cell walls.

Western blotting showed marked reduction in total proteins in bacterial isolates incubated overnight with urea solution. These findings may explain the possible mechanisms for antibacterial activity of urea solution as a result of protein denaturation.

Enrollment: 90 patients

Sex: All

Ages: 5 months to 65 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• ocular infection

Exclusion criteria

• Local antibiotics treatment

Trial design

90 participants in 1 patient group

Antibacterial Activity of Urea

Description:

Activity of Urea Solution against Ocular Bacterial Isolates

Treatment:

Biological: Antibacterial Activity