Antibacterial Effectiveness of Two Root Canal Irrigants

University of Oslo

Status and phase: Completed; Phase 2

Conditions: Periapical Periodontitis

Treatments: Procedure: Retreatment of root-filled teeth with infection

Study type: Interventional

Funder types: Other

Identifiers

NCT02747082

2012/364/REK

Details and patient eligibility

About

The aim of this randomized clinical trial is to compare the antibacterial effects of 1% sodium hypochlorite (NaOCl) and 2% chlorhexidine gluconate (CHX) during retreatment of teeth with apical periodontitis (AP).

Full description

Root canal-treated teeth with AP were randomly distributed in two groups according to the irrigant used. After removal of the previous filling material, bacteriological samples were taken from the canals before (S1) and after chemomechanical preparation using either NaOCl or CHX (S2), and after calcium hydroxide medication for 2-3 weeks (S3). 16S rRNA gene-based real-time qPCR was performed to detect and quantify total bacteria, Streptococcus species and Enterococcus faecalis.

Enrollment: 49 patients

Sex: All

Ages: 20 to 95 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Clinical diagnosis of apical periodontitis in a root-filled tooth assessed by radiography.

Exclusion criteria

• Presence of gross carious lesions in the diagnosed tooth,
• Presence of fractures involving the periodontium of the diagnosed tooth,
• Presence of periodontal pockets more than 4 mm deep in the diagnosed tooth,
• Diagnosis of diabetes,
• Diagnosis of HIV infection,
• Other immunocompromising disease,
• Antibiotic therapy within the previous 3 months.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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49 participants in 2 patient groups

1% sodium hypochlorite (NaOCl)

Active Comparator group

Description:

Retreatment of root-filled teeth with infection with 1% NaOCl as irrigation solution. Total bacterial counts, Streptococcal species and Enterococcus faecalis species were quantified using qPCR against 16SrRNA before irrigation (S1), after irrigation (S2), and after intracanal medication (S3).

Treatment:

Procedure: Retreatment of root-filled teeth with infection

2% chlorhexidine gluconate (CHX)

Active Comparator group

Description:

Retreatment of root-filled teeth with infection with 2% CHX as irrigation solution. Total bacterial counts, Streptococcal species and Enterococcus faecalis species were quantified using qPCR against 16SrRNA before irrigation (S1), after irrigation (S2), and after intracanal medication (S3).

Treatment:

Procedure: Retreatment of root-filled teeth with infection