Antimicrobial Activity of Two Mouthwashes

Universidade do Vale do Sapucai

Status: Completed

Conditions: Periimplantitis; Dental Plaque; Periodontal Diseases; Caries; Oral Surgical Wound Infection

Treatments: Drug: Sterile saline solution; Drug: Polyhexamethylene biguanide; Drug: chlorhexidine

Study type: Interventional

Funder types: Other

Identifiers

NCT02375178

MCAS antisseptico oral

Details and patient eligibility

About

This is a three arm randomized trial. The aim is to evaluate the antimicrobial activity of chlorhexidine and polyhexamethylene biguanide oral antiseptics on the microorganisms of the oral cavity. A total of 30 healthy volunteers will be enrolled and randomly allocated to control group (CG, n=10), which will be instructed to rinse the mouth with 10ml of a sterile saline solution for one minute, to chlorhexidine group (ClG, n=10), which will be instructed to rinse the mouth with 10ml of an 0.12% chlorhexidine solution, for one minute, or to polyhexamethylene biguanide group (PG, n=10), which will be instructed to rinse the mouth with 10ml of an 0.07% polyhexamethylene biguanide solution, for one minute. Samples of saliva will be collected before the mouth wash and after 30, 60 and 180 minutes. Samples will be plated on manitol agar, mitis salivarius agar, EMB agar and Sabouraud agar. Samples will be processed by a blinded microbiologist.

Enrollment: 30 patients

Sex: All

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• patients aged 18-30 years

Exclusion criteria

• patients with a hypersensitivity or allergic response by some component of the formula antiseptics,
• pregnant women,
• lactating,
• history of periodontal disease,
• smoking,
• decompensated diabetics,
• patients undergoing chemotherapy,
• radiotherapy patient,
• transplanted patient and refuses to participate,
• not signing a term of free and informed concentiment

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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30 participants in 3 patient groups

Sterile saline solution

Active Comparator group

Description:

sterile Saline solution 0,9%

Treatment:

Drug: Sterile saline solution

chlorhexidine

Experimental group

Description:

chlorhexidine 0,12% mouthwash

Treatment:

Drug: chlorhexidine

Drug: Sterile saline solution

polyhexamethylene

Experimental group

Description:

polyhexamethylene biguanide 0,07% mouthwash

Treatment:

Drug: Polyhexamethylene biguanide

Drug: Sterile saline solution