Antimicrobial Photodynamic Therapy With Erythrosine and Blue Light on Dental Biofilm Bacteria

University of Nove de Julho

Status and phase: Not yet enrolling; Phase 2; Phase 1

Conditions: Dental Plaque

Treatments: Procedure: Antimicrobial Photodynamic Therapy; Procedure: Conventional Treatment

Study type: Interventional

Funder types: Other

Identifiers

NCT05805761

EritroBiofilm

Details and patient eligibility

About

The objective of this study is to investigate the effect of Antimicrobial Photodynamic Therapy mediated by Erythrosine and blue LED in the reduction of bacteria in dental biofilm. This clinical trial will be carried out with 30 patients who have biofilm, but without the presence of periodontal pockets, and who are being treated at the Dental Clinic of Universidade Metropolitana de Santos. A split-mouth model will be used (n = 30), with group I control - conventional treatment and group II - conventional treatment and antimicrobial photodynamic therapy (aPDT). The bicarbonate jet will be used to remove dental biofilm in both groups. The treatment will be carried out in 1 session. aPDT will be performed before cleaning/prophylaxis, only in group II. Participants will rinse with the photosensitizer erythrosine (diluted to 1mM) for 1 min of pre-irradiation time, so that the drug can stain all the bacterial biofilm. Then, the D-2000 LED (DMC) will be applied, emitting at a wavelength of ʎ = 430-490 nm and 900-1100 mW/cm2. Irradiation will be performed until the biofilm of the cervical region is illuminated for 2 min per point. Each irradiation point will be approximately 0.4 cm2. The microbiological examination will be performed from samples of supragingival biofilm collected from the gingival sulcus. Two collections will be performed in each experimental site before irradiation, and immediately after the irradiation procedure (group II) or prophylaxis (groups I and II). Biofilm samples will be collected before and after exposure, respectively, and sent to the microbiological laboratory for colony counting. After this period, the CFU's will be counted and the data will be submitted to statistical analysis for comparison of pre and post-treatment results and between groups (conventional X aPDT).

Enrollment: 30 estimated patients

Sex: All

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• The participant must present between 25% and 50% of biofilm index;
• Have at least 20 teeth present, without clinical probing depths greater than 3 mm.

Exclusion criteria

• Smokers;
• Uncontrolled diabetics or hypertensives;
• Cancer or its treatment;
• Pregnant women;
• Use of antibiotics in the last 6 months;
• Coagulation disorders (use of anticoagulants, presence of liver diseases, thrombocytopenia and immunosuppression);
• Patients undergoing orthodontic treatment.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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30 participants in 2 patient groups

Conventional Treatment Group

Experimental group

Description:

Participants in this group will receive the conventional treatment for the removal of dental biofilm (prophylaxis with bicarbonate jet).

Treatment:

Procedure: Conventional Treatment

aPDT + Conventional Treatment Group

Experimental group

Description:

Participants in this group will receive both the antimicrobial photodynamic therapy and the conventional treatment (prophylaxis with bicarbonate jet) for the removal of dental biofilm.

Treatment:

Procedure: Antimicrobial Photodynamic Therapy

Procedure: Conventional Treatment