Application of Nanopore Adaptive Sequencing

Southern University of Science and Technology

Status: Begins enrollment this month

Conditions: Liver Transplant Infection

Treatments: Diagnostic Test: NAS, NGS and Laboratory tests

Study type: Observational

Funder types: Other

Identifiers

NCT06542042

SZSY001

2022YFC2304700 (Other Grant/Funding Number)

Details and patient eligibility

About

Infection post liver transplantation is an important factor in the death in patients. The traditional method of diagnosing infection post liver transplantation is laboratory tests. But the sensitivity and specificity of blood tests is poor. Next-generation sequencing (NGS) has greater detection rate for mycobacterium tuberculosis, anaerobes and fungi and greater sensitivity compare with blood tests. However use of NGS is limited because of the short read-length. Oxford nanopore adaptive sequencing (NAS) method is the Third Revolution in Sequencing Technology. For each 1 Gbp of data, NAS sequencing detected 45 times more microbiome sequences than Nanopore standard sequencing and 2.5 times more than Illumina sequencing. The purpose of this study is to compare NAS with NGS and laboratory tests for the diagnostic rate of infection post liver transplantation.

Full description

Based on the previous work, the investigators found that: 1. NAS has a higher microbial enrichment efficiency and can detect pathogenic information more quickly compared with nanopores normal sequencing (NNS); 2. NAS can detect more specific pathogen fragments with short-time sequencing; 3. NAS can cover more pathogenic genomes than NNS in a short-time; 4. NAS can detect antibiotic resistance information of pathogenic bacteria in clinical samples.

Enrollment: 100 estimated patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• Accept liver transplantation
• Signing informed consent voluntarily
• Possessing ability to comprehend material information
• Participating this study voluntarily

Exclusion criteria

• Participated another study
• Graft loss
• Have undergone a multi-organ transplantion or have had a previous organ transplantation
• Patient died

Trial design

100 participants in 3 patient groups

NAS group

Description:

In this group, the investigators will detect the number of pathogen in alveolar lavage fluid, blood and peritoneal drainage by using NAS.

Treatment:

Diagnostic Test: NAS, NGS and Laboratory tests

NGS group

Description:

In this group, the investigators will detect the number of pathogen in alveolar lavage fluid, blood and peritoneal drainage by using NGS.

Treatment:

Diagnostic Test: NAS, NGS and Laboratory tests

Lab Tests group

Description:

In this group, the investigators will detect the number of pathogen in alveolar lavage fluid, blood and peritoneal drainage by using laboratory tests.

Treatment:

Diagnostic Test: NAS, NGS and Laboratory tests