Assesment of the Functional Activity of Platelets for the Differential Diagnosis of Thrombocytopathies

Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus

Status: Not yet enrolling

Conditions: Thrombocytopathy; Acute Leukemia; Immune Thrombocytopenia; Hemorrhagic Disorders

Treatments: Diagnostic Test: Platelet aggregation and plasma coagulation; Diagnostic Test: Flow cytometry

Study type: Observational

Funder types: Other

Identifiers

NCT05654766

IBCE_PL1

Details and patient eligibility

About

The purpose of this study is to develop a highly sensitive method for assessing the functional activity of platelets for use in the differential diagnosis of thrombocytopenia and platelet defects.

Full description

The purpose of this study is to develop a highly sensitive method for assessing the functional activity of platelets using flow cytometry and turbidimetric and impedance aggregometry for use in the differential diagnosis of thrombocytopenia and platelet defects

Enrollment: 200 estimated patients

Sex: All

Ages: 1 to 17 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Pediatric participants with hemorrhagic syndrome (hemorrhagic rash, bruising, bleeding, increased bleeding of the skin and mucous membranes, spontaneous hemorrhages in soft tissues and joints)
• Pediatric participants with acute leukemia
• Parameters of the plasma level of blood coagulation correspond to the age normal levels

Exclusion criteria

• Anticoagulation drug therapy
• Antiaggregation drug therapy
• Biologically active additives

Trial design

200 participants in 2 patient groups

Control group

Description:

Group of healthy adult and pediatric participants, whose blood platelets will be used to evaluate normal ranges of parameters in the flow cytometry PFT.

Treatment:

Diagnostic Test: Platelet aggregation and plasma coagulation

Diagnostic Test: Flow cytometry

Hemorrhagic syndrome and Acute Leukemia

Description:

Group of pediatric participants with any manifestations of hemorrhagic syndrome and/or acute leukemia

Treatment:

Diagnostic Test: Platelet aggregation and plasma coagulation

Diagnostic Test: Flow cytometry