Assessment of Platelet Storage Lesion(PSL)in Platelet Concentrates

Assiut University

Status: Not yet enrolling

Conditions: Platelet Storage Lesion(PSL)

Study type: Observational

Funder types: Other

Identifiers

NCT05144074

platelet storage lesion

Details and patient eligibility

About

The aim of this study to assess platelet activation during 5 days storage of platelet concentrates prepared by different techinques .

Full description

Platelet transfusion is a lifesaving procedure that is carried out to prevent bleeding or stop ongoing bleeding in patients with low platelet count or functional platelet disorders. There are minimum thresholds at which platelets are transfused in these patients, as not all low levels of platelet warrants a transfusion. Platelet is a scarce resource as processing, preparing, and transfusing it requires a great deal of precision and effort to maintain a certain quality. This activity highlights the use of platelet transfusion by the interprofessional team (1).

Platelets play an integral role in hemostasis by its response to vascular injury. The relevance of platelet component therapy was better understood in the 1950s and 1960s when severe and fatal hemorrhagic complications of chemotherapy in leukemia were studied

The collected blood units were randomly assigned to two groups:

1. Non-filtered platelet concentrates (NF-PC group).
2. leukocyte-depleted platelet concentrates (LD-PC group). P-selectin (CD62p) is a type of glycoprotein stored in the Weibel-Palade bodies of vascular endothelial cells and platelet α-granules. Activated CD62P is a transmembrane protein expressed on the platelet surface. During platelet activation, the expression of CD62P on the surface of the cell membrane is dramatically increased in PCs without leukocyte depletion, due to the presence of WBCs and cytokines ,and is accompanied by an increase in the expression level of plasma soluble CD62

Enrollment: 180 estimated patients

Sex: All

Ages: 18 to 60 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Donors who met criteria of Egyptian National Transfusion services
• Donors who had not taken any antiplatelet drugs for two weeks prior to donation
• pre-collection platelet count ≥200×109 /L.
• Blood grouping.
• Serological assays for all blood donors (HBsAgs, HCV antibodies, HIV Ag/Ab Syphilis antibodies) on Architect i 2000 SR or Centaur XPT (chemoluminescence).

Exclusion criteria

• Donors who not met criteria of Egyptian National Transfusion services
• Donors who had taken any antiplatelet drugs for two weeks prior to donation
• pre-collection platelet count <200×109 /L.
• Reactive serology

Trial design

180 participants in 2 patient groups

Non-filtered platelet concentrates (NF-PC group).

Description:

In NF-PC group, 90 whole blood units were collected with Tripple blood bags, Six NF-PCs were pooled together and stored in a horizonal shaker at 20 ± 2C for 5 days .

leukocyte-depleted platelet concentrates (LD-PC group).

Description:

In LD-PC group, another 90 whole blood units were collected using Whole Blood Filter Saving Platelets (WB-SP) bags with an integrated leukoreduction filter