Assessment of Pulmonary Involvement inUlcerative Colitis by Induced Sputum

Hillel Yaffe Medical Center

Status: Completed

Conditions: Ulcerative Colitis

Treatments: Procedure: Induced sputum (inhalation of 3.5% saline)

Study type: Interventional

Funder types: Other

Identifiers

NCT00490048

ISUC.CTIL

Details and patient eligibility

About

Ulcerative colitis is a systemic disease we assume that extra intestinal involvement as we describein our study( Fireman Z, Osipov A, Kivity S, Kopelman Y, Sternberg A Fireman E: Assessment of pulmonary involvement in Crohn's disease by induced sputum. Am J Gastroenterol 2000;95(30):730-734. )

Full description

Twenty Ulcerative colitis patients and 20 control subjects (all nonsmokers) without respiratory symptoms will be tested. The induce sputum by 20' inhalation of 3.5% saline using ultrasonic nebulizer. Samples will studied by differential counts of 200 cells on cytopreps stained by Giemsa. T-lymphocyte subset analyses were done by FACS using three monoclonal antibodies: CD3 = total T cells, CD4 = T helper cells, and CD8 = T suppressor-cytotoxic cells. CD4/CD8 >2.5 was considered abnormal.

Enrollment: 20 estimated patients

Sex: All

Ages: 18 to 75 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• All the ulceratvive colitis patients without lung involvmentage 18-75 years of age

Exclusion criteria

• Pregnant women
• Non cooperative patients
• Smoker
• Lung disease

Trial design

Primary purpose: Screening

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)