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Assessment of Telomerase Activity in Endometrial Tissue and Serum in Endometriosis Patients

I

Istanbul University

Status

Completed

Conditions

Endometriosis Ovaries
Infertility, Female

Treatments

Procedure: Cystectomy
Procedure: Ovarian tissue sampling
Procedure: Endometrial sampling
Procedure: Peripheric blood sampling

Study type

Interventional

Funder types

Other

Identifiers

Details and patient eligibility

About

The goal of the study was the detection of telomerase activity in eutopic, ectopic endometrial tissue and in peripheral blood and finding the correlation between the telomerase activity and clinic findings. With these results, confirmation of the hypothesis regarding the endometriosis pathogenesis and endometriosis-related infertility was aimed.

Full description

Although distorted pelvic anatomy, impaired peritoneal, humoral and cellular functions, decreased implantation rate, oocyte and embryo quality, obstructed utero tubal connection are most researched aspects of endometriosis-related infertility, etiopathogenesis of endometriosis-related infertility still remains controversial.

Endometrial cell is a unique eukaryotic somatic cell with cyclic telomerase activity. Hormone-dependent cyclic variation of endometrial telomerase activity was stated in previous studies. On the other hand telomerase, activity was shown in endometriotic lesions, whereas this activity lacks in most of the somatic cells.

In this study, assessment of possible relation to increased telomerase activity in infertile patients with endometriosis was aimed.

Before published studies were assessed telomerase activity in benign and malign gynecological conditions and in endometriosis tissue, as well. But evaluation of ectopic and eutopic endometrial tissue and serum telomerase activity in infertile and fertile endometriosis and healthy control subjects was not performed.

Correlation of telomerase activity and endometriosis-related infertility was aimed in this study.

Enrollment

47 patients

Sex

Female

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Clinical diagnosis of Infertility Clinical and histological diagnosis of endometriosis

Exclusion criteria

Malign disorders Hormone replacement treatment Oral Contraceptive using

Trial design

Primary purpose

Basic Science

Allocation

Non-Randomized

Interventional model

Parallel Assignment

Masking

None (Open label)

47 participants in 3 patient groups

Infertile endometriosis
Experimental group
Description:
Cystic wall segment from cystectomy material, endometrial sampling during the surgery and peripheric blood sampling during intravenous catheterization during anesthesia.
Treatment:
Procedure: Peripheric blood sampling
Procedure: Endometrial sampling
Procedure: Cystectomy
Fertile endometriosis
Experimental group
Description:
Cystic wall segment from cystectomy material, endometrial sampling during the surgery and peripheric blood sampling during intravenous catheterization during anesthesia.
Treatment:
Procedure: Peripheric blood sampling
Procedure: Endometrial sampling
Procedure: Cystectomy
Control
Other group
Description:
Ovarian tissue sampling, endometrial sampling from hysterectomy and oophorectomy pieces and peripheric blood sampling during intravenous catheterization during anesthesia.
Treatment:
Procedure: Peripheric blood sampling
Procedure: Ovarian tissue sampling
Procedure: Endometrial sampling

Trial contacts and locations

1

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Data sourced from clinicaltrials.gov

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