Assisted Hatching in Frozen Blastocysts

Wolfson Medical Center (WMC)

Status: Unknown

Conditions: ART

Treatments: Procedure: Assisted hatching

Study type: Interventional

Funder types: Other

Identifiers

NCT03833869

0020-19-WOMC

Details and patient eligibility

About

The current study aims to assess the effect of assisted hatching on the implantation rate of frozen blastocytes.

Full description

The Zona Pellucida protects the ovum from the penetration of multiple sperm, and from mechanical and physiological damage until the fertilized ovum reaches the uterus. Subsequently, spontaneous hatching occurs, and enables the embryo to attach to the endometrium. The technique of assisted hatching was originally investigated as a means to improve implantation in cases of zona hardening, and is commonly performed in 3-day embryos. In the current study, the investigators aim to investigate the effect of assisted hatching on 5-day frozen embryos.

Enrollment: 84 estimated patients

Sex: Female

Ages: 18 to 39 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• In-vitro fertilization patients at the investigators' institution, intended to undergo a transfer of a frozen five-day embryo (blastocyte)
• 18 to 39 years old
• First to third treatment cycle
• Patient has previously had a maximum of four embryos transferred.

Exclusion criteria

• Over 40 years old
• Congenital or acquired uterine malformations
• Hydrosalpinx
• Chronic autoimmune diseases
• Embryo intended to be undergo preimplantation genetic diagnosis

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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84 participants in 2 patient groups

Assisted hatching

Experimental group

Description:

Five-day frozen embryos will undergo assisted hatching prior to embryo transfer

Treatment:

Procedure: Assisted hatching

Control

No Intervention group

Description:

Five-day frozen embryos will not undergo any additional procedures prior to embryo transfer