Association Between SNP of SP-D and Susceptibility and Prognosis of Infectious Keratitis (ABSOSASAPOIK)

Zhejiang University

Status

Completed

Conditions

Infectious Keratitis

Treatments

Diagnostic Test: corneal scraping

Other: Sequencing

Drug: Treatment of bacterial keratitis

Drug: Treatment of viral keratitis

Drug: Treatment of fungal keratitis

Study type

Observational

Funder types

Other

Identifiers

NCT04213313

2019-309 (Other Grant/Funding Number)

IR2019001224

Details and patient eligibility

About

This study explores the association between single nucleotide polymorphism (Met11Thr) of surfactant protein D(SP-D) and susceptibility and prognosis of infectious keratitis.Met11Thr of SP-D of patients with keratitis and normal controls were compared.Allele and genotype frequencies of patients with keratitis and normal controls were determined by polymerase chain reaction with sequence specific primers(PCR-SSPs) .SP-D gene polymorphism of patients with keratitis and normal controls was detected by Sanger sequencing

Full description

Surfactant protein D(SP-D) plays an important role in the innate immune system of the eye in the lacrimal duct, cornea and conjunctiva.The single lipopolysaccharide on the surface of bacteria and the receptors on the surface of immune cells are linked by SP-D ,which acts as a bridge to neutralize viruses in the innate immune defense system and assists other immune cells in clearing bacteria and fungi.SP-D can also participate in the acquired immune response after combining with lipopolysaccharide on the surface of microorganisms.Some single nucleotide polymorphisms of SP - D can not only affect the plasma level of SP - D, which leads to the differences of pathogen defense capability, but also can affect the space configuration and structure of carbohydrate recognition domain of SP-D, causing the change of ability of SP - D combined with pathogens to influence its defensive role.The concentration of SP - D in ocular surface is closely related to the occurrence and progression of infectious keratitis.Association between single nucleotide polymorphisms of SP-D and the occurrence and development of infectious keratitis has yet to be confirmed by research.

Enrollment

145 patients

Sex

All

Ages

18 to 75 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Clinical diagnosis of Infectious Keratitis.
Laboratory diagnosis of Infectious Keratitis.

Exclusion criteria

Patients with topical or systemic long-term use of hormones, interferon, or immunosuppressive therapy.
Patients with basic diseases such as diabetes, autoimmune disease, sjogren's syndrome, blepharitis, eyelid defect or incomplete eyelid closure.
Patients with history of corneal myopia laser, cataract surgery and vitrectomy, or history of serious ocular trauma and drug allergies.