Autologous Oral Mucosa Transplantation for Limbal Stem Cell Deficiency

Klinikum Chemnitz gGmbH

Status: Completed

Conditions: Limbal Stem-cell Deficiency

Study type: Observational

Funder types: Other

Identifiers

NCT03226015

EK-BR-64/15-1

Details and patient eligibility

About

PURPOSE: To report clinical and histochemical results of oral mucosa graft transplantation in eyes with limbal stem cell deficiency.

DESIGN: Prospective observational study.

METHODS: 32 eyes of 27 patients with limbal stem cell deficiency underwent direct oral mucosa graft transplantation with amniotic membrane transplantation with a mean follow-up of 19 months. Clinical course of the disease including emergency surgeries, planned curative procedures, conjunctival inflammation, acute inpatient treatment and best corrected visual acuity were assessed at 3 months postoperatively and at last follow up visit. The unneeded parts of oral mucosa graft were analyzed immunohistochemically with staining for mesenchymal stem cell markers and pericytes (CD 90, CD 146, CD 166, CD 31, CD 68, protein gene product).

Enrollment: 27 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• limbal stem cell deficiency at least in one eye
• undergoing oral mucosa with amniotic membrane transplantation
• obtained written informed consent

Exclusion criteria

• no written informed consent