Basal Instincts: Towards Better Understanding of Basal Cell Function in Chronic Rhinosinusitis With Nasal Polyps

Universitaire Ziekenhuizen KU Leuven

Status: Enrolling

Conditions: Allergic Rhinitis; Chronic Rhinosinusitis With Nasal Polyps

Treatments: Procedure: Conchotomy, aesthetic nose surgery or functional endoscopic sinus operation

Study type: Observational

Funder types: Other; Industry

Identifiers

NCT06129552

S67788

Details and patient eligibility

About

During this project, the investigators want to explore in vitro changes in basal cells and the crosstalk with residing immune cells as potential pathogenic mechanisms in CRSwNP vs healthy controls by using surgically resected patient samples.

Full description

The investigators want to use patient and healthy control samples to study/compare the following aspects in vitro:

1. Investigate differences in epithelial and basal cell functions and differentiation characteristics.
2. Characterize the differences in epithelial cell populations and gene expression patterns. Also include an AR subset, to see if changes are specific for CRSwNP or more general for type 2 inflammatory disease of the upper airways.
3. Investigate basal cell activation via different environmental triggers through TLR stimulation.
4. Look at the genetic imprinting of basal cells before and after being exposed to specific triggers.
5. Investigate whether the secreted proteins from basal cells are chemotactic for other cell populations.
6. Investigate the interaction between basal and regulatory T cells.

Enrollment: 300 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients over the age of 18
• Males and females

Exclusion criteria

• CRSwNP
• AR
• Smoker, or < 1-year ex-smoker
• Underlying systematic pathology (Morbus Wegener or Churg Strauss Syndrome for example)

Trial design

300 participants in 3 patient groups

Healthy controls

Treatment:

Procedure: Conchotomy, aesthetic nose surgery or functional endoscopic sinus operation

CRSwNP patients

Treatment:

Procedure: Conchotomy, aesthetic nose surgery or functional endoscopic sinus operation

AR patients

Treatment:

Procedure: Conchotomy, aesthetic nose surgery or functional endoscopic sinus operation