Basic Research Study to Examine the Relation Between Muscle Insulin Resistance and Lipid Production in the Liver.

Yale University

Status: Completed

Conditions: Insulin Resistance

Treatments: Other: one bout of 45 minutes exercise

Study type: Interventional

Funder types: Other; NIH

Identifiers

NCT02196051

0710003207

R56AG023686 (U.S. NIH Grant/Contract)

Details and patient eligibility

About

The purpose of this study was to examine the pathophysiology of insulin resistance and the relation to the metabolic syndrome in humans.

Full description

In this study insulin resistant (IR), young offspring of parents with type 2 diabetes (18-46 years) and healthy, lean elderly individuals (65-90 years) underwent 1H/13C MRS measurements of liver and muscle lipid and glycogen content before and after a high carbohydrate meal challenge. Hepatic de novo lipogenesis was determined as the incorporation of deuterium, from deuterium labeled water (2H2O), into plasma triglycerides 5. These metabolic studies were performed in 2 sets: Study Part 1: Resting, Study Part 2: After 1 bout of 45 minutes exercise.

The study has been completed and published as two papers: August 16, 2011 and July 24, 2012.

Enrollment: 41 patients

Sex: All

Ages: 18 to 90 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Body Mass Index < 29 kg/m2
• No regular exercise routine
• Non-smokers
• Absence of systemic and organ disease.

Exclusion criteria

• Hematocrit < 35 vol%
• History of claustrophobia
• Ferromagnetic implants
• Regular exercise regimen
• Not weight stable
• Any significant hematological, neurological, musculoskeletal or endocrine disease (including diabetes mellitus)
• Any significant cardiovascular condition, including confirmed CAD and prior cardiovascular events
• Taking coumadin as anticoagulant therapy.

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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41 participants in 1 patient group

Exercise Training

Experimental group

Description:

Participants engaged in a single bout of elliptical exercise for 45 minutes (3 times 15 minutes spaced by 5 minutes rest) at baseline. Subjects will be studied before and after this one bout to measure hepatic de novo lipogenesis. Hepatic de novo lipogenesis will be compared pre and post to examine whether acutely improving muscle glucose uptake will decrease hepatic de novo lipogenesis as the glucose is taken up by muscle and not directed to the liver.

Treatment:

Other: one bout of 45 minutes exercise