Biofilm Formation and Antibiotic Sensitivity Patterns

Sohag University

Status: Unknown

Conditions: Orthopedic Surgery

Treatments: Diagnostic Test: swab

Study type: Observational

Funder types: Other

Identifiers

NCT05181163

Soh-Med-21-11-03

Details and patient eligibility

About

Detection of bacteria that form biofilm and its sensitivity to antibiotics in patients with surgical site infection after orthopedic surgery.

Full description

The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to the following steps to identify the causative bacteria:

• Culturing on blood agar, chocolate agar, MacConkey agar and Mannitol salt agar , gram staining and biochemical tests.
• Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation.
• Antimicrobial Susceptibility Testing using the Kirby-Bauer disk diffusion method.

Enrollment: 100 estimated patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• patients with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...)

Exclusion criteria

• if patient has no orthopedic surgery