Biological Markers Evaluation of Accelerated Tooth Movement

University of Sao Paulo

Status: Unknown

Conditions: Cytokine Measurements; Tooth Movement; Orthodontic Space Closure

Treatments: Procedure: micro-osteoperforation

Study type: Interventional

Funder types: Other

Identifiers

NCT02887547

Fattori2

Details and patient eligibility

About

Gingival Crevicular Fluid samples will be collected using periopapers after accelerated tooth movement technique, results will be assessed with biomarkers levels done using ELISA Assays

Full description

For this prospective study, 22 individuals were selected, with skeletal discrepancies randomly divided into two groups: 1 - accelerated tooth movement technique (ATMT) and 2 - Control.

Orthodontic records done in two times, initial and preoperative; Gingival Crevicular Fluid samples will be collected using periopapers in 7 times (1, 2, 7, 14, 28 days and after space closure- end of orthodontic treatment movement activation).

Flapless cortical perforations will be made in ATMT Group after dental extraction. Surgical protocol will be flapless to preserve periodontal health.

After extraction space closure, results will be assessed by clinical effects with biological markers levels:

CCL3/MIP-1 alpha, CCL5/RANTES, CXCL8/IL8, IL-1 alpha, IL-1 beta, IL6, TNF-alpha, TRANCE/RANK L, OCN OPG IL-10 VEGF TGF-ℬ

Those biomarkers levels will be performed using ELISA Assays. After collecting data, the results will be statistically analyzed using Shapiro-Wilk and, posteriorly, T-student or Wilcoxon.

Enrollment: 25 patients

Sex: All

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Adult subjects ages 18-40 years old, in good general health, with adult complete dentition, regardless of presence of third molars.
• Indication for orthognathic surgery
• Tooth extraction indication for orthodontic treatment
• Do not smoke
• Periodontal and teeth healthy
• Do not use bisphosphonates or corticosteroids

Exclusion criteria

• Surgically assisted rapid palatal expansion indication
• Cleft lip and palate
• Craniofacial syndrome
• Mental disorder
• Metabolic diseases

Trial design

Primary purpose: Diagnostic

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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25 participants in 2 patient groups

Control

No Intervention group

Description:

Control group, with traditional orthodontic mechanics for anterior retraction. Crevicular fluid will be collected during anterior retraction mechanics to identified biomarkers in the inflammatory process

Acceleration

Experimental group

Description:

Group with micro-osteoperforation for accelerated tooth movement during anterior retraction, Crevicular fluid will be collected during anterior retraction mechanics to identified biomarkers in the inflammatory process

Treatment:

Procedure: micro-osteoperforation