Biomarkers in Samples From Young Patients With Acute Myeloid Leukemia

Children's Oncology Group

Status

Completed

Conditions

Leukemia

Treatments

Other: laboratory biomarker analysis

Genetic: mutation analysis

Genetic: gene expression analysis

Other: flow cytometry

Study type

Observational

Funder types

NETWORK

NIH

Identifiers

NCT01138306

AAML10B5

NCI-2011-02223 (Registry Identifier)

CDR0000671462 (Other Identifier)

COG-AAML10B5 (Other Identifier)

Details and patient eligibility

About

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about biomarkers related to cancer.

PURPOSE: This research study is studying biomarkers in samples from young patients with acute myeloid leukemia.

Full description

OBJECTIVES:

Determine the extent and prognostic implications of variable s-SHIP expression and SHIP gene mutations in pediatric patients with acute myeloid leukemia.
Determine the effects of aberrant s-SHIP expression on the PI3K/Akt pathway in these patients via electrochemiluminescence and phosphospecific flow cytometry assays.

OUTLINE: RNA samples are analyzed for variable s-SHIP expression and are screened for SHIP mutations. Cryopreserved cells (with known high- or low-s-SHIP expression) are analyzed for the levels of various components of the PI3K/Akt pathway via electrochemiluminescence and phosphospecific flow cytometry.

Enrollment

149 estimated patients

Sex

All

Ages

Under 21 years old

Volunteers

No Healthy Volunteers

Inclusion and exclusion criteria

DISEASE CHARACTERISTICS: