Biomarkers of Meats and Potatoes Intake (MEPO)

Professor Lars Ove Dragsted

Status: Completed

Conditions: Nutrition Disorders

Treatments: Other: Starchy foods arm; Other: Meat arm

Study type: Interventional

Funder types: Other

Identifiers

NCT04548362

H-150204011H

Details and patient eligibility

About

This cross-over meal study is a four-way intervention with 12 volunteers randomized at the same time to four meals with fried meats (beef, pork, chicken and amino-acid matched non-meat control) and to four meals with potato products (boiled, chips, fries, and a control with boiled white rice) to search for biomarkers of intake.

Full description

In a two-dimensional, cross-over meal study, 12 healthy volunteers consumed in a randomized sequence four test meals: chicken, pork, beef, and a control made of egg white and pea. At the same time they were independently randomized to four starchy foods to be consumed together with the meats: boiled potatoes, fried potatoes, potato crisps or boiled white rice. Fasting and postprandial urine samples were collected to cover 48 h after each meal and samples were profiled by untargeted LC-ESI-qTOF-MS metabolomics. The profiles following the meal challenges were explored by univariate and multivariate analyses and single and combined markers of intake identified chemically by MS/MS fragmentation experiments and statistically by ROC curves and error rates as well as FDR statistics.

Enrollment: 12 patients

Sex: All

Ages: 18 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• healthy

Exclusion criteria

• non-pregnant, no abuse of alcohol or drugs, no chronic medication except p-pills

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Factorial Assignment

Masking: None (Open label)

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12 participants in 2 patient groups

Meat meals

Experimental group

Description:

This arm contains a 4-way cross-over intervention study with meats

Treatment:

Other: Meat arm

Starchy meals

Experimental group

Description:

This arm contains a 4-way cross-over intervention study with

Treatment:

Other: Starchy foods arm