Blastocyst Culture in a Time-lapse Incubator

Dar AlMaraa Center

Status: Unknown

Conditions: Sub-optimal Culture Conditions

Treatments: Procedure: Culture media renwal

Study type: Interventional

Funder types: Other

Identifiers

NCT02778217

006734

Details and patient eligibility

About

Culture media used to support development of zygotes to the blastocyst stage is based on two basic philosophies: a single step medium "let-the-embryo-choose" or a sequential medium "back-to-nature".

For single step media, there are two types of protocol can be used for the culture of human preimplantation embryos:

1. Interrupted single medium culture, in which the medium is renewed on the Day 3 of embryo culture
2. Uninterrupted single embryo culture, in which the same single medium is used throughout the 5-6 days of culture with no replenishment or replacement on Day 3.

Investigators aim to compare the blastocyst development and clinical outcome between the two types of protocols of a single medium (with or without renewal on Day 3)

Enrollment: 200 estimated patients

Sex: Female

Ages: 19 to 39 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• female partners were under the age of 39 years
• Intracytoplasmic sperm injection (ICSI) was performed using fresh ejaculates

Exclusion criteria

• Day 3 embryo transfer cycles

Trial design

200 participants in 2 patient groups

Uninterrupted single embryo culture

No Intervention group

Description:

The same single medium is used throughout the 5-6 days of culture with no replenishment on day 3.

Interrupted single medium culture

Experimental group

Description:

The single step medium is renewed on Day 3 of embryo culture.

Treatment:

Procedure: Culture media renwal