Blood and Muscle Response to Cheddar Cheese in Healthy Adults

Utah System of Higher Education (USHE)

Status: Completed

Conditions: Healthy

Treatments: Dietary Supplement: Cheddar Cheese; Dietary Supplement: Milk

Study type: Interventional

Funder types: Other

Identifiers

NCT04660877

110963

Details and patient eligibility

About

The goal of this project is to use a cross over design in young healthy male and female adults:

1. To determine the amino acid blood response to the acute ingestion of cheddar cheese in comparison to that of milk.
2. To determine the muscle mTORC1 response to the acute ingestion of cheddar cheese in comparison to that of milk.

Full description

Each subject will take part in two metabolic studies. Each metabolic study was designed to test the acute blood and muscle response to an ingested amount of either cheddar cheese or milk. Approximately, one month after the first experiment (Metabolic Study #1), the participant will complete the second experiment (Metabolic Study #2) which was exact in design and at the same time of day as the first study but the participant ingested the alternate food product. The morning of the metabolic studies, the participant will arrive at the clinical research center after a 10h fast. A catheter will be placed in the participants' arm for blood sampling. Next, the participant will undergo a fasted thigh skeletal muscle biopsy. Following the muscle biopsy, the participant will consume either cheddar cheese (65g) or milk (370 mL) each amounting to 20g of protein. Subsequent muscle biopsies will occur at 60 and 180min after product ingestion. Blood sampling will occur in the fasted state and periodically after ingestion of the products (up to 300min).

Enrollment: 42 patients

Sex: All

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age between 18-35y

Exclusion criteria

1. History of cardiovascular disease
2. History of endocrine or metabolic disease (e.g., hypo/hyperthyroidism, diabetes)
3. History of kidney disease or failure
4. History of liver disease
5. History of respiratory disease (acute upper respiratory infection, chronic lung disease)
6. History of stroke with motor disability
7. Use of anticoagulant therapy (e.g., Coumadin, heparin) including aspirin and fish oils within 7d of the first biopsy experiment
8. Elevated systolic blood pressure >150 or a diastolic blood pressure > 100
9. Smoking
10. Recent anabolic or corticosteroids use (within 12 weeks)
11. Pregnancy as determined by a pregnancy test
12. self report of lactose intolerance
13. Any other medical condition or event considered exclusionary by the PI and faculty physician

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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42 participants in 2 patient groups

Cheddar chese

Experimental group

Description:

Once enrolled, participants will either ingest 20 grams of protein from 1) cheese or 2) milk for the first Metabolic Study and then the other product when they return after the washout period (\~1 month) for Metabolic Study #2.

Treatment:

Dietary Supplement: Cheddar Cheese

Milk

Active Comparator group

Description:

Once enrolled, participants will ingest 20 grams of protein from 1) cheese or 2) milk for the first Metabolic Study and then the other product when they return after the washout period (\~1 month) for Metabolic Study #2.

Treatment:

Dietary Supplement: Milk