Brassica Intake and Isothiocyanate Absorption

Beltsville Human Nutrition Research Center (BHNRC)

Status

Completed

Conditions

Healthy Volunteers

Treatments

Other: Controlled diet without brassica vegetables

Other: Controlled diet with brassica vegetables

Study type

Interventional

Funder types

Other U.S. Federal agency

Identifiers

NCT02346812

HS49

Details and patient eligibility

About

Healthy individuals (n=18) will participate in 2 controlled-feeding study periods that are 3 weeks each in length: 1) Control diet 2) Brassica diet. The control diet will consist of typical American foods and will be free of Brassica vegetables and free of glucosinolates and isothiocyanates. The Brassica diet will contain the control diet plus glucosinolate/isothiocyanate treatment foods. There will be a break of 3 weeks in between study periods. Blood, urine, and fecal samples will be collected at the end of each study period. Eligible participants will be selected based on genotype for glutathione S-transferase (GST), which has been shown to influence the potential protective role of dietary Brassicas. Half the participants (n=9) will be GSTM1-positive individuals and half (n=9) will have the GSTM1-null genotype.

Enrollment

18 patients

Sex

All

Ages

21 to 70 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Between 21 - 70 years of age
Not currently taking glucosinolate/ITC containing supplements
Voluntarily agree to participate and sign an informed consent document
Free of cancer (never diagnosed or cancer-free for at least 5 y)

Exclusion criteria

Pregnant, lactating, or intend to become pregnant during the study period
Known allergy or intolerance to Brassica vegetables
History of bariatric surgery or nutrient malabsorption disease (such as celiac disease) or other metabolic disorders requiring special diet recommendations
Use of tobacco products
Use of certain medications (prescription or over-the-counter) that may interfere with the study objectives
Type 2 diabetes requiring the use of diabetes pills, insulin, or non-insulin shots
Use of blood-thinning medications such as Coumadin (warfarin), Dicumarol, or Miradon (anisindione)
Unable or unwilling to give informed consent or communicate with study staff
Self-report of alcohol or substance abuse within the past 12 months and/or current acute treatment or rehabilitation program for these problems (long-term participation in Alcoholics Anonymous is not an exclusion)
Other medical, psychiatric, or behavioral factors that in the judgment of the Principal Investigator may interfere with study participation or the ability to follow the intervention protocol