Cardiomyocyte Apoptosis Following Antegrade and Retrograde Cardioplegia

Turku University Hospital (TYKS)

Status: Completed

Conditions: Cardiomyocyte Apoptosis

Treatments: Procedure: Ventricle muscle tissue samples

Study type: Observational

Funder types: Other

Identifiers

NCT01185912

1/2006

Details and patient eligibility

About

Retrograde cardioplegia during heart surgery is associated with inadequate myocardial protection. In experimental animal study retrograde cardioplegia induces more cardiomyocyte apoptosis when compared to antegrade cardioplegia. 20 volunteer patients under going elective aortic valve surgery will be divided in to two groups: 10 patients receiving only antegrade cardioplegia and 10 patients receiving only retrograde cardioplegia. Pre- and post-operatively cardiac MRI and cardiac ultrasound will be performed. During the surgery ventricular muscle samples will be taken in order to assess cardiomyocyte apoptosis. Aims of the study: 1. whether the cardiomyocyte apoptosis is significantly more induced after retrograde cardioplegia than antegrade cardioplegia, 2. whether the amount of apoptotic cardiomyocytes correlates to conventional markers of myocardial injury after cardiac surgery, 3. whether the extent of irreversible myocardial damage by apoptosis correlates to post-ischemic contractile dysfunction after cardiac surgery, as measured with echocardiography, 4. whether the amount of cardiomyocyte apoptosis correlates to long term outcome and cardiac function as measured with MRI.

Enrollment: 20 patients

Sex: All

Ages: 60 to 90 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• aortic valve stenosis or combined aortic valve disease

Exclusion criteria

• angiographically verified hemodynamically significant coronary artery disease impaired left ventricular function (left ventricular ejection fraction <50%) surgery including additional procedures other than aortic valve replacement cancer

Trial design

20 participants in 1 patient group

Cardiomyocyte apoptosis

Description:

Elective aortic valve replacement patience

Treatment:

Procedure: Ventricle muscle tissue samples