Cell-free DNA: a Non-invasive Test for Assessing Embryo Quality

IRCCS Azienda Ospedaliera Universitaria San Martino - IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy

Status: Completed

Conditions: Culture Media; DNA

Study type: Observational

Funder types: Other; Industry

Identifiers

NCT01397136

GFI2011_PS

Details and patient eligibility

About

In the present project we propose to deeply characterize nuclear and/or mitochondrial DNA in embryo culture medium, in order to investigate whether both cell-free nucleic acid types may provide additional, reliable, predictive parameters for embryo viability and implantation potential.

Enrollment: 600 patients

Sex: All

Ages: 2 to 7 days old

Volunteers: No Healthy Volunteers

Inclusion criteria

• We will enroll in the study only embryos obtained from fresh gametes. It is to note that we currently transfer more than one embryo (with a maximum of 3, according to patient's age) and we will follow such criteria alongside the project. Consequently, we will take into consideration this aspect in correlating DNA parameters with embryo's outcome.

Exclusion criteria

• We will exclude cryopreserved embryos, in order to avoid potential biases due to cooling/defrosting procedures.