Changes in the Distribution of Mitochondria During Oocyte Maturation

Hillel Yaffe Medical Center

Status: Unknown

Conditions: Infertility

Treatments: Other: Fixation and staining; Other: Observation by laser microscopy

Study type: Observational

Funder types: Other

Identifiers

NCT01501292

0095-11-HYMC

Details and patient eligibility

About

Cytoplasmatic maturation is an important event in the general maturation of of the oocyte. Mitochondria is one of the major organelles involved in the process of cytoplasmatic maturation. The aim of this study is to investigate the presence and distribution of the mitochondria in the cytoplasma of immature oocytes that are retrieved in the routine in-vitro maturation cycle with intracytoplasmatic sperm injection (ICSI). These oocytes will not undergo ICSI because of their immaturity. Usually, they are discarded. In addition to these immature oocytes the investigators shall also investigate the mitochondria in mature oocytes that underwent ICSI but did not become fertilized and are discarded.

Enrollment: 20 estimated patients

Sex: Female

Ages: 18 to 44 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Infertile patients needing IVF treatment

Exclusion criteria

• Patients not requiring the ICSI procedure

Trial design

20 participants in 2 patient groups

Immature oocytes (GV, M-I)

Treatment:

Other: Observation by laser microscopy

Other: Fixation and staining

Mature oocytes (M-II)

Treatment:

Other: Observation by laser microscopy

Other: Fixation and staining