Changes in the Immunomodulatory Properties of Adipose Stromal Cells Due to Hemarthrosis After Knee Injury (CASH)

Radboud University Medical Center

Status

Enrolling

Conditions

Knee Injuries

Post-Traumatic Osteoarthritis of Knee

Hemarthrosis

Treatments

Procedure: venipuncture

Diagnostic Test: Physical examination

Procedure: knee arthrocentesis

Diagnostic Test: MRI-scan

Other: KOOS questionnaire

Study type

Observational

Funder types

Other

Identifiers

NCT05687331

NL77974.091.21

Details and patient eligibility

About

The goal of this observational study is to gain insight in the natural course of pro-inflammatory factors and hemarthrosis in patients older than 18 with a recent knee trauma. The main question[s] it aims to answer are:

What is the natural course of pro-inflammatory factors and hemarthrosis in knee trauma?
What are the effects of inflammation and hemarthrosis on the anti-inflammatory potential of ASC's in order to better determine eligible patients and circumstances for ASC's therapy?

Participants will:

undergo blood withdrawal
undergo knee arthrocentesis for synovial fluid sample collection
physical examination
fill out a questionnaire on knee complaints

Full description

Recent studies have found an amelioration of post-traumatic osteoarthritis by the use of adipose-derived stromal cells (ASC's) in an experimental animal model. This amelioration was only seen under inflammatory conditions. The presence of hemarthrosis has not been taken into account in the before mentioned animal model, and might have cytotoxic effects on ASC's and aggravate the inflammatory response. The objective of this study is to gain insight in the natural course of pro-inflammatory factors and hemarthrosis and its effects on the anti-inflammatory potential of ASC's in order to better determine eligible patients and circumstances for ASC's therapy.

Therefore the investigators designed a pilot study with exploratory analyses in and with synovial fluid in a longitudinal cohort.

Our study population will consist of 20 patients, older than 18 years, who sustained a recent knee trauma and are presented to the emergency department.The investigators will gather synovial fluid and blood samples for laboratory analysis perform and physical examination and provide questionnaires for clinical follow-up.

The main study endpoint are the anti-inflammatory capacities of ASC's in synovial fluid that contains blood as a result of hemarthrosis after knee injury.

Enrollment

20 estimated patients

Sex

All

Ages

18+ years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Recently suffered an injury of the index knee (rotational or hyperextension)
A swollen joint
Inability to mobilise

Exclusion criteria

Patients with an active inflammatory or infectious comorbid disease (including rheumatic diseases)
Patients using systemic immunosuppressant medication
Patients with a contra-indication for undergoing a MRI-scan (non-compatible implants or claustrophobia for example)
Patients with knee prosthesis
Patients with coagulation disorders
Patients with a history of a cruciate ligament or meniscal injury of the index knee
Patients with a tibiofemoral fracture of the index knee due to current distortion
In case the inclusion of the required amount of patient is reached in one the patient groups, additional patients belonging in the same patient group will not be included in the study