Childhood Metabolic Markers of Adult Morbidity in Blacks
Status
Conditions
Details and patient eligibility
About
Blacks are at increased risk for obesity, type 2 diabetes mellitus and cardiovascular disease. A common pathogenetic link among these entities is insulin resistance/hyperinsulinemia.
The specific aims of this project are: 1) to compare skeletal muscle lipid content (SMLC) in black vs white children by computed tomography (CT) scan of the mid-thigh, and assess the relationship to in vivo insulin sensitivity; 2) to test the hypothesis that free fatty acid (FFA) - induced insulin resistance is associated with larger increases in intramyocellular lipid (IMCL) in black vs white adolescents; 3) to examine if β-cell insulin secretion in prepubertal black children is more sensitive to the stimulatory effect of FFA than in whites; and 4) to test if the β-cell in black obese adolescents is more susceptible to the lipotoxic effect of FFA compared with whites. The methods to be used are: the well- established CT method as well as Magnetic Resonance Spectroscopy (1H-MRS) to assess SMLC and IMCL; intralipid infusion to elevate circulating FFA levels; the hyperinsulinemic-euglycemic clamp with stable isotopes and indirect calorimetry to measure insulin sensitivity and substrate turnover; the hyperglycemic clamp to assess insulin secretion; DEXA and whole body MRI for body composition assessments.
Enrollment
Sex
Ages
Volunteers
Inclusion criteria
Protocols 1 & 4:
- Age 11-17 years
- Male or Female
- Healthy
- Obese, BMI ≥ 95 percentile
- Pubertal/Tanner Stage II-V
- African American or White American, based on self identity with no admixture for 3 generations
Protocol 2:
- Age 11-17 years
- Male or Female
- Healthy
- Normal Weight, BMI 10- 95 percentile
- Pubertal/Tanner Stage II-V
- African American or White American, based on self-identity with no admixture for 3 generations
Protocol 3:
- Age 8-12 years
- Male or Female
- Healthy
- Normal Weight, BMI 10-95 percentile
- Prepubertal/Tanner Stage I
- African American or White American, based on self-identity with no admixture for 3 generations
Exclusion criteria
- Medications which interfere with metabolism
- Hemocue < 12 gm/dl in pubertal subjects and <11gm/dl in prepubertal subjects
- Positive serum pregnancy test
- Recent significant weight change or dieting
- Presence of disease (i.e.diabetes, hypothyroidism, genetic dyslipidemia, etc)
Trial design
156 participants in 4 patient groups
Trial contacts and locations
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Data sourced from clinicaltrials.gov
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