Circulating Levels of Irisin in Healthy Young Subjects (IRISSAS)

424 General Military Hospital

Status: Completed

Conditions: Healthy Subjects

Treatments: Dietary Supplement: Boost; Other: Aerobic exercise

Study type: Observational

Funder types: Other

Identifiers

NCT01986530

SSAS-1-MANTZ

424-IRIS (Other Identifier)

Details and patient eligibility

About

Irisin, a newly discovered myokine induced in exercise, has potential effects in stimulating adipose tissue browning, fighting obesity and diabetes. No prior study has reported on the role of circulating irisin in healthy individuals in correlation with lean and fat body mass. Furthermore, the circadian and seasonal variation of irisin is largely unknown.

Full description

The main aims of this prospective study will be to investigate: 1) circulating irisin levels in healthy, young men and women according to their lean and fat mass; 2) the circadian variations of irisin levels; 3) the seasonal variations of irisin levels.

Enrollment: 122 patients

Sex: All

Ages: 18 to 25 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion Criteria: Healthy volunteers

Exclusion Criteria: i) body mass index (BMI) above 30 or below 20 kg/m2; ii) diseases that could affect muscle or fat homeostasis; iii) medications that could affect muscle or fat homeostasis.

Trial design

122 participants in 5 patient groups

Healthy individuals

Description:

Participants will be healthy volunteers of both sexes recruited from the Greek Military Medical School personnel, Thessaloniki, Greece.

Boost Subgroup

Description:

After an overnight fast, 40 participants will be provided a standardized mixed meal in two different quantities (125 ml, n=20 and 250 ml, n=20) and blood samples will be obtained before as well as 30 min after mixed meal ingestion

Treatment:

Dietary Supplement: Boost

Aerobic exercise

Description:

After an overnight fast, 20 participants will be subjected to aerobic exercise for 30 min and blood samples will be obtained at baseline and at 30 min

Treatment:

Other: Aerobic exercise

Circadian variation Subgroup

Description:

20 of the participants will be hospitalized and closely monitored for 24 hours. A catheter will be inserted in a vein and blood samples will be obtained every 3 hours through the 24-hour period.

Seasonal variation Subgroup

Description:

20 of the participants will be monitored for one year and blood samples will be obtained every 3 months (at the middle of month January - April - July - October). Subjects will be instructed to maintain their normal exercise routine and dietary habits.