Circulatory Levels of Irisin in Response to Acute Resistance Training

Texas Tech University

Status: Completed

Conditions: Healthy

Treatments: Other: exercise training

Study type: Interventional

Funder types: Other

Identifiers

NCT02478658

504792A

Details and patient eligibility

About

Study the circulatory levels of irisin in response to one session of high-intensity resistance training in a group of healthy subjects.

Full description

Objective: The purpose of this study is to study the circulating levels of irisin in response to acute high-intensity resistance training in a group of healthy young adults.

Sample: The sample will be comprised of adult men and women unpaid volunteers between the ages of 18 and 30. We will recruit 44 participants.

Pre- screening assessments: Informed consent, PAR-Q, Medical History and Exercise History will be used to determine health status and prior exercise experience. Menstrual cycle log will be required for female participants.

Pre-Intervention assessments (baseline):

• Anthropometric measurements and Body Composition: Height, triceps, thigh and calf skinfolds, biceps, thigh, hip, waist, and calf circumferences will be measured. Dual-energy x-ray absorptiometry (DEXA) will be performed to obtain body composition.
• Blood-related variables. Baseline glucose, lactate and hematocrit will be obtained from all participants prior to intervention. Lactate and Hematocrit will be obtained pre-intervention and post-intervention, and within 1 min post workout.

Irisin will be obtained from blood samples at baseline, during and after the training session. Irisin will be measured via a commercial enzyme-linked immunosorbent assay.

• Cardiopulmonary and Strength Fitness Testing. Cardiopulmonary fitness will be determined via maximum oxygen consumption testing. One maximum repetition (1-RM) will be used to assess the muscular fitness.
• Dietary Controls. Each participant will complete the Food Preference Questionnaire. In addition, the Automated Self-Administered 24-hour Recall (ASA24) will be collected for the day prior to the session.
• Familiarization. Participant will be instructed on the muscle conditioning techniques used for strength training so that the techniques can be mastered. The rate of perceived exertion (RPE) scale will also be used for familiarization during these sessions.
• Spontaneous Physical Activity: Daily activity level for 7 days will be collected.

Intervention: Resistance training will consist on a circuit training of 3 sets of 7 exercises focused on major muscle groups. Ten repetitions on each circuit at 70% of the maximum capacity will be performed. Acute intervention will consist of one session. Blood will be collected pre-session, at 45 min during the session, and immediately after the acute intervention exposure (post-session).

Enrollment: 28 patients

Sex: All

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Participants between 18 and 30 years old
• Males and Females
• Healthy

Exclusion criteria

• Participants with known health problems such as cardiovascular, pulmonary or metabolic disease will be excluded.
• Pregnant females will be excluded from the study.
• Any adults found taking medications that affect endocrine or cardiovascular function will also be excluded from the study.
• Individuals found to be less than 110 pounds will also be excluded due to the collection of blood during this study.
• Participants that exercise more than three times per week or strength train more than twice per week will be excluded.
• Individuals that have poor venous access or have expressed a fear of needles or having blood taken will also be excluded.
• Cigarette smoking (current or those who quit within the previous 6 months
• Hypertension
• Overweight and obese
• Pre-diabetics

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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28 participants in 2 patient groups

Intervention

Experimental group

Description:

The acute intervention will consist of 1 session of training following the program: Resistance Training Exercises. Each participant in the intervention group will perform resistance-training exercises in the form of a circuit. The circuit will consist of 10 repetitions per exercise of 7 exercises: leg press, bent-over row, bench press, squats, dumbbell jump squats with raises, dead-lifts and weighted abdominal crunches, with approximately 30 sec of rest in between each exercise (based on the estimated time needed to move from one position to the next). Initial intensity 6-7 of RPE and ending the set at 9-10. Each participant will move through the circuit 3 times, with 2 to 3 minutes of rest between each round.

Treatment:

Other: exercise training

Control

No Intervention group

Description:

No intervention