Combined Whey Protein and Collagen Supplementation in Resistance-Trained Men

Ankara Yildirim Beyazıt University

Status: Completed

Conditions: Bone Mineral Density Loss; Muscle Hypertrophy; Resistance Training Adaptation

Treatments: Dietary Supplement: Collagen Peptides; Dietary Supplement: Whey Protein; Other: Maltodextrin (Placebo); Dietary Supplement: Whey Protein + Collagen

Study type: Interventional

Funder types: Other

Identifiers

NCT07171411

IAU.Ilk.C.162911421.2024

Details and patient eligibility

About

This study tested whether taking whey protein together with collagen peptides would provide greater benefits for muscle and bone health compared to whey protein alone, collagen alone, or a placebo. Forty healthy, resistance-trained men aged 18-35 years were randomly assigned to one of four groups: (1) whey protein plus collagen (30 g + 10 g/day), (2) whey protein only (30 g/day), (3) collagen only (10 g/day), or (4) placebo (maltodextrin). All participants followed a supervised resistance training program (3 times per week) for 8 weeks.

The primary outcome was muscle mass, measured with bioelectrical impedance analysis (BIA). Secondary outcomes included bone mineral density (DXA scans), maximal strength (1RM squat and bench press), and blood markers of bone turnover (P1NP and CTX-I).

Results showed that the whey + collagen group achieved the largest improvements in muscle growth, lumbar spine bone mineral density, strength, and favorable changes in bone turnover markers compared to all other groups. No serious side effects were reported, and supplement adherence was very high.

These findings suggest that combining whey protein and collagen may be a practical strategy to support muscle and bone adaptation in resistance-trained men.

Full description

This was a double-blind, randomized, placebo-controlled trial evaluating the combined effects of whey protein and collagen supplementation on muscle mass, bone mineral density (BMD), muscular strength, and bone turnover markers in resistance-trained men.

Forty healthy male participants (18-35 years, with ≥1 year of resistance training experience) were recruited and randomized into four parallel groups (n=10 per group):

Whey Protein + Collagen (30 g WP + 10 g collagen/day)

Whey Protein only (30 g/day)

Collagen only (10 g/day)

Placebo (10 g maltodextrin/day, isocaloric)

All supplements were provided in identical single-dose sachets to ensure blinding. Participants consumed one dose upon waking and one post-exercise (or same time on rest days). A standardized, supervised hypertrophy-oriented resistance training program (3 sessions/week for 8 weeks) was applied to all groups.

Primary outcome: Muscle mass (lean body mass, assessed with BIA at baseline and 8 weeks).

Secondary outcomes: Lumbar spine and femoral neck BMD (DXA), maximal squat and bench press strength (1RM), and serum biomarkers of bone turnover (P1NP and CTX-I).

The trial was approved by the Islamic Azad University IRB (Protocol No: IAU.Ilk.C.162911421.2024). All participants gave written informed consent. Compliance with supplementation was >90% and training adherence was >95%. No adverse events were reported.

Enrollment: 40 patients

Sex: Male

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Male participants aged 18-35 years
• At least 1 year of consistent resistance training experience (≥3 sessions per week, targeting major muscle groups)
• Healthy, with no history of musculoskeletal, metabolic, or cardiovascular disorders
• No current or recent (<6 months) use of anabolic steroids, growth hormones, or performance-enhancing substances
• Non-smoker and no habitual use of anti-inflammatory medications
• Able and willing to comply with supplementation and supervised training program
• Provided written informed consent

Exclusion criteria

• Participation in similar supplementation studies in the past 6 months
• Recent fractures or surgeries affecting musculoskeletal health (<6 months)
• Known allergy or intolerance to dairy proteins or collagen supplements
• Inability to attend scheduled supervised resistance training sessions
• Non-adherence to dietary control requirements (e.g., use of additional protein/creatine supplements during study)
• Any condition deemed by investigators to interfere with study compliance or safety

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Quadruple Blind

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40 participants in 4 patient groups, including a placebo group

Whey Protein + Collagen (WP+C)

Experimental group

Description:

Participants received 30 g/day whey protein isolate plus 10 g/day type I \& III collagen peptides, split into two daily doses (morning and post-exercise or at same time on rest days). Supplements were packaged in identical sachets.

Treatment:

Dietary Supplement: Whey Protein + Collagen

Whey Protein Only (WP)

Active Comparator group

Description:

Participants received 30 g/day whey protein isolate, split into two daily doses. Packaged identically to other interventions to maintain blinding.

Treatment:

Dietary Supplement: Whey Protein

Collagen Only (C)

Active Comparator group

Description:

Participants received 10 g/day type I \& III collagen peptides, split into two daily doses. Identical packaging ensured blinding.

Treatment:

Dietary Supplement: Collagen Peptides

Placebo (P)

Placebo Comparator group

Description:

Participants received 10 g/day maltodextrin (isocaloric with protein groups), provided in identical sachets to ensure blinding.

Treatment:

Other: Maltodextrin (Placebo)