Comparative Analysis of Salivary and Bronchoalveolar Lavage IL-6 and TNF-α as Biomarkers in Pediatric Acute Respiratory Distress Syndrome

Ain Shams University

Status: Invitation-only

Conditions: ARDS (Acute Respiratory Distress Syndrome)

Treatments: Diagnostic Test: Bronchoscopy

Study type: Observational

Funder types: Other

Identifiers

NCT07380373

MD88/2025

Details and patient eligibility

About

The aim of this study is to determine the degree of agreement in cytokines level measured through BAL and those measured through salivary glands excretion.

and to compare the validity of cytokines in BAL versus salivary secretion in predicting the stage of ARDS and disease outcome in term of mortality, disease progression and length of stay.

Full description

• BAL will be performed by the attending pediatric intensivist in pediatric intensive care unit as part of standard care for ARDS management and Saliva samples will be collected non-invasively using sterile collection devices IL6 and TNF alfa will measured in both samples and compared to each other

Enrollment: 37 estimated patients

Sex: All

Ages: 1 to 10 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Clinical diagnosis of Pediatric Acute Respiratory Distress Syndrome (ARDS)
• Age range: 1 month to 18 years
• Requirement for invasive mechanical ventilation
• Availability of salivary and bronchoalveolar lavage (BAL) samples within 24 hours of ARDS diagnosis
• Informed consent obtained from parent or legal guardian

Exclusion criteria

• Pre-existing chronic lung disease (e.g., cystic fibrosis, bronchopulmonary dysplasia)
• Known immunodeficiency or current immunosuppressive therapy
• Active malignancy or receipt of chemotherapy within the past 6 months
• Severe congenital anomalies incompatible with survival
• Refusal or inability to obtain informed consent